Hebei Key Laboratory of Applied Chemistry, School of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao 066004, China.
Hebei Key Laboratory of Applied Chemistry, School of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao 066004, China; State Key Laboratory of Metastable Materials Science and Technology, Yanshan University, Qinhuangdao 066004, China.
Biosens Bioelectron. 2018 Feb 15;100:1-7. doi: 10.1016/j.bios.2017.07.077. Epub 2017 Aug 15.
In this work, a novel ultrasensitive electrochemical biosensor was developed for the detection of K562 cell by a signal amplification strategy based on multiple layer CdS QDs functionalized polystyrene microspheres(PS) as bioprobe and graphene oxide(GO) -polyaniline(PANI) composite as modified materials of capture electrode. Due to electrostatic force of different charge, CdS QDs were decorated on the surface of PS by PDDA (poly(diallyldimethyl-ammonium chloride)) through a layer-by-layer(LBL) assemble technology, in which the structure of multiple layer CdS QDs increased the detection signal intensity. Moreover, GO-PANI composite not only enhanced the electron transfer rate, but also increased tumor cells load ratio. The resulting electrochemical biosensor was used to detect K562 cells with a lower detection limit of 3 cellsmL (S/N = 3) and a wider linear range from 10 to 1.0 × 10 cellsmL. This sensor was also used for mannosyl groups on HeLa cells and Hct116 cells, which showed high specificity and sensitivity. This signal amplification strategy would provide a novel approach for detection, diagnosis and treatment for tumor cells.
在这项工作中，通过基于多层 CdS QDs 功能化聚苯乙烯微球（PS）作为生物探针和氧化石墨烯（GO）-聚苯胺（PANI）复合材料作为捕获电极修饰材料的信号放大策略，开发了一种用于检测 K562 细胞的新型超灵敏电化学生物传感器。由于不同电荷的静电力，CdS QDs 通过 PDDA（聚二烯丙基二甲基氯化铵）通过层层（LBL）组装技术被修饰在 PS 的表面上，其中多层 CdS QDs 的结构增加了检测信号强度。此外，GO-PANI 复合材料不仅增强了电子转移速率，而且增加了肿瘤细胞的负载率。所得电化学生物传感器用于检测 K562 细胞，检测限低至 3 个细胞 mL（S/N = 3），线性范围从 10 到 1.0×10 个细胞 mL 较宽。该传感器还用于检测 HeLa 细胞和 Hct116 细胞上的甘露糖基，表现出高特异性和灵敏度。这种信号放大策略将为肿瘤细胞的检测、诊断和治疗提供一种新方法。
