Jaiswal Shilpi, Kundu Subhankar, Bandyopadhyay Sujoy, Patra Abhijit
Department of Chemistry, Indian Institute of Science Education and Research, Bhopal Bhopal Bypass Road, Bhauri Bhopal 462066 Madhya Pradesh India
Nanoscale Adv. 2021 Apr 1;3(11):3232-3239. doi: 10.1039/d0na00995d. eCollection 2021 Jun 1.
We fabricated an inorganic-organic hybrid upconversion nanoprobe for the ratiometric detection of aliphatic biogenic amines in water. The hybrid nanoprobe comprises a thiophene-based acceptor-π-donor-π-acceptor organic fluorescent dye, TDPM, and near-infrared light-absorbing upconversion nanoparticles (UCNPs). The organic dye was loaded into a mesoporous silica-coated UCNP (UCNP@mSiO) matrix to circumvent the issues of water insolubility and higher energy excitation. Yb and Tm-doped UCNPs exhibited dual emission bands at 475 and 645 nm upon excitation with a 980 nm laser. The significant spectral overlap between the absorption and the emission bands of TDPM and UCNPs, respectively, at 475 nm led to resonance energy transfer (RET) from the UCNPs to TDPM resulting in the quenching of the UCNP emission. In contrast, 'turn-on' emission was noticeable with the addition of aliphatic biogenic amines due to an inhibition of the RET. The emission at 645 nm remained unaffected during the energy transfer process making the hybrid probe a versatile platform for the ratiometric detection of different aliphatic biogenic amines. Furthermore, we explored the sensing of aliphatic biogenic amines in adulterated milk and rotten fish. The unique material attributes demonstrated in the current study hold promise for further development of real-time sensors and switches based on hybrid upconversion nanoprobes.
我们制备了一种无机-有机杂化上转换纳米探针，用于水中脂肪族生物胺的比率检测。该杂化纳米探针由基于噻吩的受体-π-供体-π-受体有机荧光染料TDPM和近红外光吸收上转换纳米颗粒（UCNPs）组成。将有机染料负载到介孔二氧化硅包覆的UCNP（UCNP@mSiO）基质中，以解决水不溶性和高能激发的问题。用980nm激光激发时，掺镱和铥的UCNPs在475和645nm处呈现双发射带。TDPM和UCNPs在475nm处的吸收带和发射带之间存在显著的光谱重叠，导致从UCNPs到TDPM的共振能量转移（RET），从而导致UCNP发射猝灭。相反，由于RET受到抑制，加入脂肪族生物胺后出现“开启式”发射。在能量转移过程中，645nm处的发射保持不变，这使得杂化探针成为检测不同脂肪族生物胺的通用比率检测平台。此外，我们还研究了掺假牛奶和腐烂鱼中脂肪族生物胺的传感。当前研究中展示的独特材料特性为基于杂化上转换纳米探针的实时传感器和开关的进一步开发带来了希望。
