Sayed Alaa El-Din H, Soliman Hamdy A M
Zoology Department, Faculty of Science, Assiut University, 71516 Assiut, Egypt.
Zoology Department, Faculty of Science, Sohag University, 8562 Sohag, Egypt.
Mutat Res Genet Toxicol Environ Mutagen. 2017 Oct;822:34-40. doi: 10.1016/j.mrgentox.2017.07.002. Epub 2017 Jul 15.
Although, silver nanoparticles (AgNPs) are used in many different products, little information is known about their toxicity in tropical fish embryos. Therefore, this study evaluated the developmental toxicity of waterborne silver nanoparticles in embryos of Clarias gariepinus. Embryos were treated with (0, 25, 50, 75ng/L silver nanoparticles) in water up to 144h postfertilization stage (PFS). Results revealed various morphological malformations including notochord curvature and edema. The mortality rate, malformations, and DNA fragmentation in embryos exposed to silver nanoparticles increased in a dose- and embryonic stage-dependent manner. The total antioxidant capacity and the activity of catalase in embryos exposed to 25ng/L silver nanoparticles were decreased significantly while the total antioxidant capacity and the activity of catalase were insignificantly increased with increasing concentrations in the embryos from 24 to 144 h-PFS exposed to 50 and 75ng/L silver nanoparticles. Lipid peroxidation values showed fluctuations with doses of silver nanoparticles. Histopathological lesions including severely distorted and wrinkled notochord were observed. The current data propose that the toxicity of silver nanoparticles in C. gariepinus embryos is caused by oxidative stress and genotoxicity.
尽管银纳米颗粒（AgNPs）被用于许多不同的产品中，但关于它们对热带鱼胚胎毒性的信息却知之甚少。因此，本研究评估了水体中银纳米颗粒对尖吻鲈胚胎的发育毒性。在受精后144小时（PFS）前，用含（0、25、50、75纳克/升银纳米颗粒）的水对胚胎进行处理。结果显示出各种形态畸形，包括脊索弯曲和水肿。暴露于银纳米颗粒的胚胎中的死亡率、畸形率和DNA片段化呈剂量和胚胎阶段依赖性增加。暴露于25纳克/升银纳米颗粒的胚胎中的总抗氧化能力和过氧化氢酶活性显著降低，而在受精后24至144小时暴露于50和75纳克/升银纳米颗粒的胚胎中，随着浓度增加，总抗氧化能力和过氧化氢酶活性增加不显著。脂质过氧化值随银纳米颗粒剂量而波动。观察到组织病理学损伤，包括严重扭曲和起皱的脊索。目前的数据表明，银纳米颗粒对尖吻鲈胚胎的毒性是由氧化应激和遗传毒性引起的。
