Liu Wei, Yang Fan, Lv Hai-Hua, Zhou Jie, Wang Xiao, Geng Yan-Ling
Key Laboratory of Traditional Chinese Medicine Quality Control Techlogy, Shandong Analysis and Test Center, Ji'nan 250014, China.
Zhongguo Zhong Yao Za Zhi. 2016 Jan;41(1):56-59. doi: 10.4268/cjcmm20160111.
This paper is aimed to study plant biomass and active compounds of Scutellaria baicalensis germchit in different five stages (from germination to transplant). The length of shoot and root, the diameter and the weight of root were determined. HPLC method was used to determine the content of active compounds (baicalin, scutellarin, wogonoside, baicalein, wogonin). According to the results, various biological indicators increased with the germination of seedling. However, the drying rate of the root declined to 27.96% from 32.90%. The contents of scutellarin and baicalein increased firstly, and then decreased. The maximums of them were 3.22,3.89 mg•g⁻¹ while the data of shoot/root was 0.35. The maximums of the contents of baicalin and wogonoside were 107.39,16.11 mg•g⁻¹ while the data of shoot/root was 0.23 and 0.06. The contents of wogonin gradually increased to the maximum of 0.88 mg•g⁻¹ while the data of shoot/root was 0.50. In conclusion, the contents of baicalin, scutellarin, wogonoside, baicalein and wogonin reached or approached the maximum at germination stage while the data of shoot/root was 0.35. The rate of shoot and root can be used as a judging index of active compounds for S. baicalensis germchit.
本文旨在研究不同五个阶段（从发芽到移栽）的黄芩种子苗的植物生物量和活性成分。测定了地上部分和根的长度、根的直径和重量。采用高效液相色谱法测定活性成分（黄芩苷、野黄芩苷、汉黄芩苷、黄芩素、汉黄芩素）的含量。结果表明，随着幼苗发芽，各项生物学指标均增加。然而，根的干燥率从32.90%下降到27.96%。野黄芩苷和黄芩素的含量先增加后降低，其最大值分别为3.22、3.89mg•g⁻¹，此时地上部分与根的比值为0.35。黄芩苷和汉黄芩苷含量的最大值分别为107.39、16.11mg•g⁻¹，此时地上部分与根的比值分别为0.23和0.06。汉黄芩素的含量逐渐增加至最大值0.88mg•g⁻¹，此时地上部分与根的比值为0.50。综上所述，黄芩苷、野黄芩苷、汉黄芩苷、黄芩素和汉黄芩素的含量在发芽阶段达到或接近最大值，此时地上部分与根的比值为0.35。地上部分和根的生长速率可作为黄芩种子苗活性成分的判断指标。
