Grossman Z, Mietz J A, Kuff E L
Nucleic Acids Res. 1987 May 11;15(9):3823-34. doi: 10.1093/nar/15.9.3823.
Poly(A)RNAs prepared from the thymuses of C57BL/6J and DBA/2J mice were used to construct cDNA libraries in the bacterial expression vector lambda gt11. The libraries were scanned first for protein production with polyvalent antiserum prepared against the 73kDa gag protein of mouse intracisternal A-particles (IAP). Reactive plaques were crossed-screened by hybridization with an IAP-specific DNA probe. Two IAP-specific protein-producing plaques were obtained from the C57BL/6 library and 4 from the DBA/2 library. One C57BL/6 cDNA clone (B12) and two DBA cDNA clones (D8 and D20) were sequenced in their entirety. Clones B12 and D8 were remarkably similar, particularly when compared to the 6 other IAP elements that have been sequenced thus far. We discuss the evidence which leads us to suggest that these clones may be derived from allelic IAP elements expressed in mouse thymus.
从C57BL/6J和DBA/2J小鼠胸腺制备的多聚腺苷酸RNA（Poly(A)RNAs）被用于构建细菌表达载体λgt11中的cDNA文库。首先用针对小鼠脑内A颗粒（IAP）的73kDa gag蛋白制备的多价抗血清对文库进行蛋白质产生的筛选。通过与IAP特异性DNA探针杂交对反应性噬菌斑进行交叉筛选。从C57BL/6文库中获得了两个产生IAP特异性蛋白的噬菌斑，从DBA/2文库中获得了4个。对一个C57BL/6 cDNA克隆（B12）和两个DBA cDNA克隆（D8和D20）进行了全长测序。克隆B12和D8非常相似，特别是与迄今为止已测序的其他6个IAP元件相比。我们讨论了使我们提出这些克隆可能源自小鼠胸腺中表达的等位基因IAP元件的证据。
