Mellor A L, Weiss E H, Kress M, Jay G, Flavell R A
Cell. 1984 Jan;36(1):139-44. doi: 10.1016/0092-8674(84)90082-5.
DNA sequence analysis of a class I gene (Q10), which maps to the Qa2,3 locus in the C57BL/10 (H-2b haplotype) mouse, reveals that it is almost identical to a cDNA clone (pH16) isolated from a SWR/J (H-2q haplotype) mouse liver cDNA library. Exon 5, in particular, has an unusual structure such that a polypeptide product is unlikely to be anchored in the cell membrane. Our findings suggest that the two sequences are derived from allelic class I genes, which are nonpolymorphic, in contrast to H-2K allelic sequences from the same mice, and they may encode liver-specific polypeptides of unknown function. Our previous studies indicate that the Q10 gene is a potential donor gene for the generation of mutations at the H-2K locus by inter-gene transfer of genetic information. Thus the lack of polymorphism in class I genes at the Q10 locus implies either that they are not recipients for such exchanges or that selective pressure prevents the accumulation of mutations in genes at this locus.
对一个I类基因（Q10）进行DNA序列分析，该基因定位于C57BL/10（H-2b单倍型）小鼠的Qa2,3位点，结果显示它与从SWR/J（H-2q单倍型）小鼠肝脏cDNA文库中分离出的一个cDNA克隆（pH16）几乎完全相同。特别是外显子5具有不寻常的结构，以至于不太可能有一个多肽产物锚定在细胞膜上。我们的研究结果表明，这两个序列源自等位I类基因，与同一小鼠的H-2K等位基因序列不同，它们是非多态性的，并且可能编码功能未知的肝脏特异性多肽。我们之前的研究表明，Q10基因是通过基因间遗传信息转移在H-2K位点产生突变的潜在供体基因。因此，Q10位点I类基因缺乏多态性意味着要么它们不是此类交换的接受者，要么选择压力阻止了该位点基因中突变的积累。
