Tavakoli Kareshk A, Mahmoudvand H, Keyhani A, Tavakoli Oliaee R, Mohammadi M A, Babaei Z, Hajhosseini M A, Zia-Ali N
Department of Medical Parasitology and Mycology, Kerman University of Medical Sciences, Kerman, Iran.
Razi Herbal Medicines, Lorestan University of Medical Scienses, Khorramabad, Iran.
Trop Biomed. 2017 Sep 1;34(3):681-690.
This study was designed to detect parasitic DNA in tissues from sheep and goats raised and slaughtered in the southeastern Iran as well as to genetically characterize infecting strains of T. gondii. A total of 240 tissue samples consisting of heart, brain, and diaphragm were obtained from sheep (n=40) and goats (n=40) slaughtered in abattoirs from three provinces located in southeastern Iran including Kerman, Razavi Khorasan, and South Khorasan Provinces between February to October 2015. Nested PCR amplified the B1 and GRA6 genes. To determine the genetic characterization of positive samples, all genotyped positive samples were examined by PCR-RFLP. Sequencing analysis was performed to evaluate the prevalence of type strains (I, II and III). A total of 68(56.66%) tissue samples of sheep and 53(44.16%) from goats were found to be positive for T. gondii B1 gene, that included 11(27.5%) diaphragm, 21(52.5%) heart, and 36(90%) brain of sheep; and 20(50%) diaphragm, 11(22%) heart and 22(55%) brain of goats. Moreover, 22(18.3%) tissue samples of sheep and 20(16.6%) tissue samples of goats were found positive with GRA6 gene for T. gondii. There are three genotypes and mix genotype using mseI enzyme among all positive samples. The results demonstrated the presence of T. gondii DNA in tissues of sheep and goats from southeast of Iran. Control of Toxoplasma infection animal products are important in consumer protection.
本研究旨在检测伊朗东南部饲养和屠宰的绵羊和山羊组织中的寄生虫DNA，并对感染的弓形虫菌株进行基因特征分析。2015年2月至10月期间，从伊朗东南部克尔曼、拉扎维霍拉桑和南霍拉桑三省的屠宰场宰杀的绵羊（n = 40）和山羊（n = 40）中，共采集了240份组织样本，包括心脏、大脑和膈肌。巢式PCR扩增B1和GRA6基因。为确定阳性样本的基因特征，所有基因分型的阳性样本均通过PCR-RFLP进行检测。进行测序分析以评估I、II和III型菌株的流行情况。共发现68份（56.66%）绵羊组织样本和53份（44.16%）山羊组织样本的弓形虫B1基因呈阳性，其中包括绵羊的11份（27.5%）膈肌、21份（52.5%）心脏和36份（90%）大脑；山羊的20份（50%）膈肌、11份（22%）心脏和22份（55%）大脑。此外，22份（18.3%）绵羊组织样本和20份（16.6%）山羊组织样本的弓形虫GRA6基因呈阳性。所有阳性样本中使用mseI酶检测到三种基因型和混合基因型。结果表明伊朗东南部绵羊和山羊组织中存在弓形虫DNA。控制动物产品中的弓形虫感染对保护消费者很重要。
