Assis Gabriella B N, Pereira Felipe L, Zegarra Alexandra U, Tavares Guilherme C, Leal Carlos A, Figueiredo Henrique C P
AQUACEN, National Reference Laboratory for Aquatic Animal Diseases, Ministry of Agriculture, Livestock and Food Supply, Federal University of Minas GeraisBelo Horizonte, Brazil.
Front Microbiol. 2017 Aug 9;8:1492. doi: 10.3389/fmicb.2017.01492. eCollection 2017.
Gram-positive cocci, such as , and subsp. , are found throughout the world, particularly in outbreaks in farmed fish, and are thus associated with high economic losses, especially in the cultivation of Nile Tilapia. The aim of this study was to evaluate the efficacy of matrix-assisted laser desorption ionization (MALDI)-time of flight (TOF) mass spectrometry (MS) as an alternative for the diagnosis of these pathogens. One hundred and thirty-one isolates from Brazilian outbreaks assisted by the national authority were identified using a MALDI Biotyper from Bruker Daltonics. The results showed an agreement with respect to identification (Kappa = 1) between this technique and 16S ribosomal RNA gene sequencing for and . However, for and subsp. , perfect agreement was only achieved after the creation of a custom main spectra profile, as well as further comparisons with 16S ribosomal RNA and multilocus sequence analysis. MALDI-TOF MS was shown to be an efficient technology for the identification of these Gram-positive pathogens, yielding a quick and precise diagnosis.
革兰氏阳性球菌，如[具体菌种1]、[具体菌种2]和[具体菌种3]亚种，在世界各地均有发现，尤其在养殖鱼类疫情中出现，因此会造成巨大经济损失，在尼罗罗非鱼养殖中尤为如此。本研究旨在评估基质辅助激光解吸电离（MALDI）-飞行时间（TOF）质谱（MS）作为诊断这些病原体的替代方法的有效性。使用布鲁克道尔顿公司的MALDI Biotyper对来自巴西疫情且由国家权威机构协助的131株分离株进行了鉴定。结果表明，该技术与[具体菌种1]和[具体菌种2]的16S核糖体RNA基因测序在鉴定方面达成了一致（Kappa = 1）。然而，对于[具体菌种3]和[具体菌种3]亚种，只有在创建自定义主谱图并与16S核糖体RNA和多位点序列分析进行进一步比较后，才实现了完全一致。MALDI-TOF MS被证明是一种用于鉴定这些革兰氏阳性病原体的有效技术，能够实现快速准确的诊断。
