Zhang Yan-Mei, Chen Min, Sun Ling, Wang Yue, Yin Jianmei, Liu Jia, Sun Xiao-Qin, Hang Yue-Yu
Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, China.
Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
Front Genet. 2020 May 7;11:484. doi: 10.3389/fgene.2020.00484. eCollection 2020.
is an important food crop that is mainly cultivated in subtropical regions of the world. is frequently infected by various pathogens during its lifespan, which results in a substantial economic loss in terms of yield and quality. The disease resistance gene ( gene) profile of is largely unknown, which has greatly hampered molecular study of disease resistance in this species. () genes are the largest group of plant genes, and they play important roles in plant defense responses to various pathogens. In this study, 167 genes were identified from the genome. Subsequently, one gene was assigned to the () () subclass and the other 166 genes to the () () subclass. None of the () () genes were detected in the genome. Among them, 124 genes are located in 25 multigene clusters and 43 genes are singletons. Tandem duplication serves as the major force for the cluster arrangement of genes. Segmental duplication was detected for 18 genes, although no whole-genome duplication has been documented for the species. Phylogenetic analysis revealed that genes share 15 ancestral lineages with genes. The gene number increased by more than a factor of 10 during evolution. A conservatively evolved ancestral lineage was identified from , which is orthologs to the gene. Transcriptome analysis for four different tissues of revealed a low expression of most genes, with the tuber and leaf displaying a relatively high gene expression than the stem and flower. Overall, this study provides a complete set of genes for , which may serve as a fundamental resource for mining functional genes against various pathogens.
是一种重要的粮食作物，主要种植在世界亚热带地区。在其生长周期中经常受到各种病原体的感染，这在产量和质量方面导致了巨大的经济损失。该作物的抗病基因（基因）图谱在很大程度上尚不清楚，这极大地阻碍了该物种抗病性的分子研究。（）基因是植物基因中最大的一组，它们在植物对各种病原体的防御反应中发挥重要作用。在本研究中，从该作物基因组中鉴定出167个基因。随后，一个基因被归类到（）（）亚类，另外166个基因被归类到（）（）亚类。在该基因组中未检测到（）（）基因。其中，124个基因位于25个多基因簇中，43个基因为单拷贝基因。串联重复是基因簇排列的主要驱动力。检测到18个基因存在片段重复，尽管该物种尚未有全基因组重复的记录。系统发育分析表明，基因与基因共享15个祖先谱系。在进化过程中，基因数量增加了10倍以上。从该作物中鉴定出一个保守进化的祖先谱系，它与基因是直系同源的。对该作物四个不同组织的转录组分析表明，大多数基因表达水平较低，块茎和叶片中的基因表达相对高于茎和花。总体而言，本研究为该作物提供了一套完整的基因，这可能作为挖掘针对各种病原体的功能性基因的基础资源。
