Marzban Mohsen, Anjamshoa Maryam, Jafari Parnia, Masoumi Hossien, Ahadi Reza, Fatehi Daryoush
Ph.D. Student of Neuroscience, Faculty of Advanced Medical Technologies, Tehran University of Medical Sciences, Tehran, Iran.
Ph.D., Assistant Professor of Anatomical Sciences, Faculty of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Electron Physician. 2017 Jun 25;9(6):4524-4532. doi: 10.19082/4524. eCollection 2017 Jun.
To determine the radioprotective effects of Silymarin in adult male Sprague-Dawley rats irradiated with γ-rays.
The present experimental study was performed in Tehran University of Medical Sciences, Tehran, Iran from December 2009 to March 2010. The study was performed on 40 rats, which were randomly and equally divided into four groups: 1) control group: neither received Silymarin nor irradiated with γ-rays; 2) γ-irradiation group: testis region exposed to 2Gy of γ-rays; 3) Silymarin & γ-irradiation: rats received 100 mg/kg of Silymarin 24hrs before exposure to 2Gy of γ-rays; 4) Silymarin & γ-irradiation: rats received 200 mg/kg of Silymarin 24hrs before exposure to 2Gy of γ-rays. After animal experiments and preparing the tissue sections, different histological and histomorphological parameters of seminiferous tubules and the biological characteristics of Leydig cells were evaluated applying quantitative assessment, Johnson scoring, and Leydig cell apoptosis assay by TUNEL method. The data were analyzed applying ANOVA and Tukey's post hoc test, using SPSS software (V.19).
Irradiation of 2 Gy γ-rays to the testis of the rats significantly affected the frequency of spermatogonia, primary spermatocyte, round spermatid, spermatozoa, seminiferous tube and lumen diameters, thickness of the epithelium, Leydig cell nuclear diameter and volume, epithelium height, and apoptotic cells (p<0.05). However, administration of Silymarin improved the mentioned parameters specifically in 200 mg/kg of dosage.
Silymarin could act as a potent radioprotector and it can be used in modulation as well as improvement to radiation therapy to prevent male reproductive function, specifically seminiferous tubules in an animal model; however, its molecular mechanism is still not clear and needs more molecular researches.
确定水飞蓟素对受γ射线照射的成年雄性斯普拉格-道利大鼠的辐射防护作用。
本实验研究于2009年12月至2010年3月在伊朗德黑兰医科大学进行。对40只大鼠进行研究，将其随机等分为四组：1）对照组：既未接受水飞蓟素也未接受γ射线照射；2）γ射线照射组：睾丸区域接受2 Gy的γ射线照射；3）水飞蓟素与γ射线照射组：大鼠在接受2 Gyγ射线照射前24小时接受100 mg/kg水飞蓟素；4）水飞蓟素与γ射线照射组：大鼠在接受2 Gyγ射线照射前24小时接受200 mg/kg水飞蓟素。动物实验并制备组织切片后，应用定量评估、约翰逊评分和TUNEL法进行的莱迪希细胞凋亡检测，评估生精小管的不同组织学和组织形态学参数以及莱迪希细胞的生物学特性。使用SPSS软件（版本19）应用方差分析和Tukey事后检验对数据进行分析。
对大鼠睾丸进行2 Gyγ射线照射显著影响精原细胞、初级精母细胞、圆形精子细胞、精子、生精小管和管腔直径、上皮厚度、莱迪希细胞核直径和体积、上皮高度以及凋亡细胞的频率（p<0.05）。然而，水飞蓟素的给药改善了上述参数，特别是在200 mg/kg剂量时。
水飞蓟素可作为一种有效的辐射防护剂，可用于调节和改善放射治疗，以预防动物模型中的男性生殖功能，特别是生精小管；然而，其分子机制仍不清楚，需要更多的分子研究。
