Liu Meihong, Liu Huimin, Xie Jiahan, Xu Qian, Pan Cong, Wang Jiaojiao, Wu Xi, Zheng Mingzhu, Liu Jingsheng
College of Food Science and Engineering, Jilin Agricultural University, Changchun, Jilin 130118, China.
Food Funct. 2017 Sep 20;8(9):3327-3338. doi: 10.1039/c7fo00486a.
Zeaxanthin, a type of carotenoid, has been proven to exhibit anti-lipogenesis effect; however, the detailed mechanism of this effect is less known. Herein, we evaluated the effects of zeaxanthin on the inhibition of adipogenesis in 3T3-L1 adipocytes and obesity in high-fat diet fed C57BL/6J mice. Zeaxanthin significantly decreased the intracellular lipid content in a dose-dependent manner (5-15 μM) in adipocytes without causing cytotoxicity. In high-fat-diet-induced obese mice, oral administration of 20 mg kg zeaxanthin attenuated the progression of obesity and improved dyslipidemia. It exhibits an anti-adipogenic effect via down-regulating the transcriptional factors and adipocyte-specific genes involved in adipogenesis, both in vitro and in vivo. Furthermore, zeaxanthin treatment reversed the MDI (0.5 mM 3-isobutyl-1-methylxanthine, 1.0 μM dexamethasone, and 1.0 μg mL insulin) and HFD (high-fat diet)-induced inhibition of AMPK phosphorylation in adipocytes and epididymal adipose tissues, respectively, thereby modulating the energy metabolism. These results indicated that zeaxanthin plays anti-adipogenic and anti-obesity roles by inducing AMPK activation, inhibiting lipogenesis, and decreasing intracellular lipid content, adipocyte size, and adipose weight.
玉米黄质是一种类胡萝卜素，已被证明具有抗脂肪生成作用；然而，这种作用的详细机制尚不清楚。在此，我们评估了玉米黄质对3T3-L1脂肪细胞脂肪生成抑制以及高脂饮食喂养的C57BL/6J小鼠肥胖的影响。玉米黄质以剂量依赖性方式（5-15μM）显著降低脂肪细胞内脂质含量，且不引起细胞毒性。在高脂饮食诱导的肥胖小鼠中，口服20mg/kg玉米黄质可减轻肥胖进展并改善血脂异常。在体外和体内，它通过下调参与脂肪生成的转录因子和脂肪细胞特异性基因发挥抗脂肪生成作用。此外，玉米黄质处理分别逆转了MDI（0.5mM 3-异丁基-1-甲基黄嘌呤、1.0μM地塞米松和1.0μg/mL胰岛素）和高脂饮食（HFD）诱导的脂肪细胞和附睾脂肪组织中AMPK磷酸化的抑制，从而调节能量代谢。这些结果表明，玉米黄质通过诱导AMPK活化、抑制脂肪生成以及降低细胞内脂质含量、脂肪细胞大小和脂肪重量发挥抗脂肪生成和抗肥胖作用。
