Department of Urology, Linyi People's Hospital, Linyi, Shandong 276003, P.R. China.
Mol Med Rep. 2017 Oct;16(4):5489-5494. doi: 10.3892/mmr.2017.7218. Epub 2017 Aug 10.
Long non-coding RNAs (LncRNAs) have been reported to serve roles in various types of malignancy, including human renal cell carcinoma (RCC), which is among the most common types of kidney cancer worldwide. The present study aimed to investigate the effects of a newly‑discovered LncRNA, Z38, on cell proliferation and metastasis in RCC cells. Reverse transcription‑quantitative polymerase chain reaction analysis was used to detect the transcription levels of Z38 in clinical RCC tissues and cultured RCC cells. The expression of Z38 was notably increased in patients with stage III and IVRCC compared with patients with stage I and II. Knockdown of Z38 with specific short hairpin RNAs notably decreased the proliferation rate of A498 and ACHIN cells. In addition, a colony formation assay was included to investigate the role of Z38 in cell proliferation. Transwell assays demonstrated that Z38 deprivation inhibited the migratory and invasive capability of RCC cells. The association between Z38 and the epithelial‑mesenchymal transition process was investigated using western blot analysis. The results of the present study demonstrated that Z38 may serve as an important biomarker in the diagnosis and treatment of RCC in the clinic.
长链非编码 RNA（lncRNAs）已被报道在各种类型的恶性肿瘤中发挥作用，包括人类肾细胞癌（RCC），这是全球最常见的肾癌类型之一。本研究旨在探讨一种新发现的 lncRNA，Z38，对 RCC 细胞增殖和转移的影响。逆转录-定量聚合酶链反应分析用于检测临床 RCC 组织和培养的 RCC 细胞中 Z38 的转录水平。与 I 期和 II 期 RCC 患者相比，III 期和 IV 期 RCC 患者的 Z38 表达明显增加。用特异性短发夹 RNA 敲低 Z38 明显降低了 A498 和 ACHIN 细胞的增殖率。此外，还进行了集落形成试验以研究 Z38 在细胞增殖中的作用。Transwell 分析表明，Z38 剥夺抑制了 RCC 细胞的迁移和侵袭能力。使用 Western blot 分析研究了 Z38 与上皮-间充质转化过程之间的关联。本研究的结果表明，Z38 可能作为临床诊断和治疗 RCC 的重要生物标志物。
