Department of Urinary Surgery, Ningbo Beilun People's Hospital, Ningbo, Zhejiang, China.
Department of General Practice, Ningbo Beilun People's Hospital, Ningbo, Zhejiang, China.
Technol Cancer Res Treat. 2021 Jan-Dec;20:1533033821997834. doi: 10.1177/1533033821997834.
Renal cell carcinoma (RCC) is a type of urinary tumor with a high incidence and is often associated with tumor metastasis. Long non-coding RNA (lncRNA) regulates tumorigenesis, progression, and metastasis. However, the role and the predictive value of lncRNA in RCC progression and metastasis have not been elucidated. The purpose of this study was to evaluate the effect of a newly discovered lncRNA LOC648987 on RCC proliferation and metastasis. LOC648987 was identified by RT-PCR for high expression in human RCC tissues as well as in metastatic RCC tissues. In the cell experiments, we infected the RCC cell lines ACHN and 786-O cells with LOC648987-shRNA and its negative control (shNC). The results showed that the knockdown of LOC648987 inhibited the proliferation of ACHN and 786-O cells and colony formation. The cell cycle and the apoptosis progression of ACHN and 786-O cells were assessed using flow cytometry. The knockdown of LOC648987 significantly inhibited the progression of ACHN and 786-O cells from G0/G1 to S phase and promoted cell apoptosis. The metastasis promoting effects of LOC648987 on ACHN and 786-O cells were verified by transwell migration assays, which depended on vimentin and MMP-9 to regulate the epithelial-mesenchymal transition. Finally, the promotion of LOC648987 on RCC tumorigenesis was evaluated in BALb/c nude mice. These data confirmed that lncRNA LOC648987 promoted RCC cell proliferation and tumor metastasis and regulated the expression of EMT-related proteins in RCC cells.
肾细胞癌（RCC）是一种高发的泌尿系统肿瘤，常伴有肿瘤转移。长链非编码 RNA（lncRNA）调节肿瘤发生、发展和转移。然而，lncRNA 在 RCC 进展和转移中的作用及其预测价值尚未阐明。本研究旨在评估新发现的 lncRNA LOC648987 对 RCC 增殖和转移的影响。RT-PCR 鉴定发现，LOC648987 在人 RCC 组织和转移性 RCC 组织中表达较高。在细胞实验中，我们用 LOC648987-shRNA 和其阴性对照（shNC）感染 RCC 细胞系 ACHN 和 786-O 细胞。结果表明，LOC648987 的敲低抑制了 ACHN 和 786-O 细胞的增殖和集落形成。用流式细胞术评估 ACHN 和 786-O 细胞的细胞周期和凋亡进程。LOC648987 的敲低显著抑制了 ACHN 和 786-O 细胞从 G0/G1 期向 S 期的进展，并促进细胞凋亡。Transwell 迁移实验验证了 LOC648987 对 ACHN 和 786-O 细胞转移的促进作用，该作用依赖于波形蛋白和 MMP-9 调节 EMT。最后，在 BALB/c 裸鼠中评估了 LOC648987 对 RCC 肿瘤发生的促进作用。这些数据证实，lncRNA LOC648987 促进了 RCC 细胞的增殖和肿瘤转移，并调节了 RCC 细胞中 EMT 相关蛋白的表达。
