Department of Urology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu 221002, P.R. China.
Mol Med Rep. 2017 Nov;16(5):6050-6058. doi: 10.3892/mmr.2017.7361. Epub 2017 Aug 24.
T‑Box (TBX)‑2 is a member of the T‑box gene family, which is aberrantly expressed in numerous types of malignant tumors, and has previously been demonstrated to be conducive to tumor progression by acting as a transcription factor. However, specific information regarding the expression and function of TBX2 in prostate cancer cells remains to be elucidated. The present study demonstrated that silencing of TBX2 by TBX2 small interfering RNA inhibited cell proliferation and promoted cell senescence. It was demonstrated that knockdown of TBX2 inhibited cell metastatic abilities by upregulating E‑cadherin and downregulating N‑cadherin, Vimentin and fibronectin. In addition, the expression of TBX2 in prostate cancer tissues and tumor adjacent tissues was detected by immunohistochemistry. The results indicated that the expression rates of TBX2 were significantly increased in the cancerous tissues, compared with the healthy tumor adjacent tissue, and TBX2 increased staining was associated with the clinical stage and pathological grade. The findings of the present study therefore suggest that TBX2 expression is markedly increased in prostate cancer and TBX2 may act as a potential beneficial therapeutic target for the future treatment of prostate cancer.
T-盒基因 2（TBX2）是 T-盒基因家族的成员，在多种恶性肿瘤中异常表达，先前的研究表明其作为转录因子有利于肿瘤的进展。然而，TBX2 在前列腺癌细胞中的表达和功能的具体信息仍有待阐明。本研究表明，通过 TBX2 小干扰 RNA 沉默 TBX2 抑制了细胞增殖并促进了细胞衰老。研究表明，通过上调 E-钙黏蛋白和下调 N-钙黏蛋白、波形蛋白和纤维连接蛋白，敲低 TBX2 抑制了细胞的转移能力。此外，通过免疫组织化学检测了 TBX2 在前列腺癌组织和肿瘤相邻组织中的表达。结果表明，与健康的肿瘤相邻组织相比，TBX2 在癌组织中的表达率显著增加，并且 TBX2 增强染色与临床分期和病理分级相关。因此，本研究的结果表明，TBX2 在前列腺癌中表达明显增加，TBX2 可能成为未来治疗前列腺癌的潜在有益治疗靶点。
