Ma Lin, Andoh Vivian, Shen Zhongyuan, Liu Haiyan, Li Long, Chen Keping
College of Biotechnology, Jiangsu University of Science and Technology Zhenjiang Jiangsu 212001 P. R. China
Institute of Life Science, Jiangsu University Zhenjiang Jiangsu 212013 P. R. China
RSC Adv. 2022 Jun 10;12(27):17276-17284. doi: 10.1039/d2ra01161a. eCollection 2022 Jun 7.
Despite many research efforts devoted to the study of the effects of magnesium oxide nanoparticles (MgO NPs) on cells or animals in recent years, data related to the potential long-term effects of this nanomaterial are still scarce. The aim of this study is to explore the subchronic effects of MgO NPs on silkworm, a complete metamorphosis insect with four development stages (egg, larva, pupa, month). With this end in view, silkworm larvae were exposed to MgO NPs at different mass concentrations (1%, 2%, 3% and 4%) throughout their fifth instar larva. Their development, survival rate, cell morphology, gene expressions, and especially silk properties were compared with a control. The results demonstrate that MgO NPs have no significant negative impact on the growth or tissues. The cocooning rate and silk quality also display normal results. However, a total of 806 genes are differentially expressed in the silk gland (a vital organ for producing silk). GO (Gene Ontology) results show that the expression of many genes related to transporter activity are significantly changed, revealing that active transport is the main mechanism for the penetration of MgO NPs, which also proves that MgO NPs are adsorbed by cells. KEGG (Kyoto Encyclopedia of Genes and Genomes) analysis demonstrates that the longevity regulating pathway-worm, peroxisome and MAPK signaling pathway are closely involved in the biological effects of MgO NPs. Overall, subchronic exposure to MgO NPs induced no apparent negative impact on silkworm growth or silks but changed the expressions of some genes.
尽管近年来人们致力于研究氧化镁纳米颗粒（MgO NPs）对细胞或动物的影响，但有关这种纳米材料潜在长期影响的数据仍然很少。本研究的目的是探讨MgO NPs对家蚕（一种具有四个发育阶段——卵、幼虫、蛹、成虫的完全变态昆虫）的亚慢性影响。为此，在整个五龄幼虫期，将家蚕幼虫暴露于不同质量浓度（1%、2%、3%和4%）的MgO NPs中。将它们的发育、存活率、细胞形态、基因表达，特别是蚕丝特性与对照组进行比较。结果表明，MgO NPs对家蚕的生长或组织没有显著的负面影响。结茧率和蚕丝质量也显示正常结果。然而，共有806个基因在丝腺（生产蚕丝的重要器官）中差异表达。基因本体论（GO）结果表明，许多与转运蛋白活性相关的基因表达发生了显著变化，这表明主动运输是MgO NPs渗透的主要机制，这也证明了MgO NPs被细胞吸附。京都基因与基因组百科全书（KEGG）分析表明，长寿调节通路——线虫、过氧化物酶体和丝裂原活化蛋白激酶（MAPK）信号通路与MgO NPs的生物学效应密切相关。总体而言，亚慢性暴露于MgO NPs对家蚕生长或蚕丝没有明显的负面影响，但改变了一些基因的表达。
