Han Binsong, Xin Zhongquan, Ma Shasha, Liu Wenbin, Zhang Bingyang, Ran Lu, Yi Lunzhao, Ren Dabing
Yunnan Food Safety Research Institute, Kunming University of Science and Technology, Kunming 650500, PR China.
School of Science, Kunming University of Science and Technology, PR China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Sep 15;1063:84-92. doi: 10.1016/j.jchromb.2017.08.021. Epub 2017 Aug 19.
Antioxidants from natural sources, such as vegetables and fruits, are attracting more and more interest. In this work, we evaluated the antioxidant potential of Folium Artemisia Argyi, a traditional Chinese herb medicine and food supplement. The total phenolic content, total flavonoid content, and antioxidant ability of the crude extracts and fractions obtained from consecutively partition of n-hexane, ethyl acetate, and n-butanol were measured and compared. Ethyl acetate fraction shows the highest total phenolic and flavonoid contents and highest antioxidant capability with regard to DPPH, ABTS, superoxide anion free radical scavenging ability, and ferric-reducing antioxidant power. In addition, the potential antioxidant components were screened by DPPH-UHPLC-MS experiments and subsequently characterized by using high-resolution tandem mass spectrometry. This work finally identified 45 antioxidants, including organic acids, phenolic compounds, flavonoids, and methoxylated flavonoids. The results suggested that Folium Artemisiae Argyi is a potential inexpensive resource of natural antioxidants.
来自蔬菜和水果等天然来源的抗氧化剂正吸引着越来越多的关注。在这项工作中，我们评估了艾草叶的抗氧化潜力，艾草叶是一种传统的中草药和食品补充剂。测定并比较了从正己烷、乙酸乙酯和正丁醇连续分配得到的粗提物和馏分的总酚含量、总黄酮含量和抗氧化能力。乙酸乙酯馏分显示出最高的总酚和黄酮含量，以及在DPPH、ABTS、超氧阴离子自由基清除能力和铁还原抗氧化能力方面的最高抗氧化能力。此外，通过DPPH-UHPLC-MS实验筛选了潜在的抗氧化成分，随后使用高分辨率串联质谱对其进行了表征。这项工作最终鉴定出45种抗氧化剂，包括有机酸、酚类化合物、黄酮类化合物和甲氧基黄酮类化合物。结果表明，艾草叶是一种潜在的廉价天然抗氧化剂资源。
