Tea Research Institute, Zhejiang University, Hangzhou, 310058, China.
Sci Rep. 2017 Aug 29;7(1):9847. doi: 10.1038/s41598-017-08587-6.
Tea plant is a typical fluorine (F) accumulator. F concentration in mature tea leaves is several hundred times higher than that in normal field crops. Long-term consumption of teas with high level F will increase the risks of dental and skeletal fluorosis. The mechanism of F accumulation in tea stands unclear. RNA-Seq and digital gene expression (DGE) techniques were used to investigate the effect of F on the differential expressions of transcriptome in tea plant. The results showed that F content in mature tea leaves was increased with increase in F concentration of cultural solution and duration of F treatment time. Based on comparison with data of GO, COG, KEGG and Nr databases, 144 differentially expressed unigenes with definite annotation were identified. Real-time reverse transcription PCR (qRT-PCR) was used to validate the effect of F on expression of 5 unigenes screened from the 144 unigenes. F treatment induced the expression of defense genes such as receptor-like kinases (RLKs) and U-box domain-containing protein. Based on the present study, F uptake is considered to be related to calcium-transporting ATPase, especially autoinhibited Ca ATPase (ACAs) which was activated by the RLKs and worked as a carrier in uptake of F by tea plant.
茶树是一种典型的氟（F）积累植物。成熟茶叶中的 F 浓度比正常田间作物高几百倍。长期饮用高氟含量的茶会增加患氟斑牙和氟骨症的风险。F 在茶树中积累的机制尚不清楚。RNA-Seq 和数字基因表达（DGE）技术被用于研究 F 对茶树转录组差异表达的影响。结果表明，随着培养液中 F 浓度和 F 处理时间的增加，成熟茶叶中的 F 含量增加。与 GO、COG、KEGG 和 Nr 数据库的数据进行比较后，鉴定出了 144 个具有明确注释的差异表达的 unigenes。实时反转录 PCR（qRT-PCR）用于验证从 144 个 unigenes 中筛选的 5 个 unigenes 的 F 处理对其表达的影响。F 处理诱导了受体样激酶（RLKs）和 U 盒结构域蛋白等防御基因的表达。基于本研究，F 的摄取被认为与钙转运 ATP 酶有关，特别是 RLKs 激活的自动抑制 Ca ATP 酶（ACAs），它作为茶树摄取 F 的载体。
