Kaneda Shohei, Kawada Jiro, Akutsu Hidenori, Ichida Justin, Ikeuchi Yoshiho, Fujii Teruo
Center for International Research on Integrative Biomedical Systems, Institute of Industrial Science, The University of Tokyo, Tokyo, Japan.
Department of Reproductive Medicine, Centre for Regenerative Medicine, National Research Institute for Child Health and Development, Tokyo, Japan.
Biomicrofluidics. 2017 Aug 2;11(4):041101. doi: 10.1063/1.4994989. eCollection 2017 Jul.
We developed a compartmentalized culture system of single embryoid bodies (EBs) utilizing a through-hole on a membrane to induce spatially patterned differentiation. An EB derived from mouse pluripotent stem cells was immobilized on the through-hole. By introducing a stem cell maintenance medium and a differentiation medium into upper and lower culture compartments, respectively, a localized differentiated state was achieved only in the lower part of EB, which is exposed to the medium in the lower compartment. This system may enable us to reconstruct complex tissues and to recapitulate developmental processes using EBs.
我们开发了一种利用膜上的通孔诱导空间模式分化的单个胚状体（EB）的分隔培养系统。将源自小鼠多能干细胞的EB固定在通孔上。通过分别向上部和下部培养隔室中引入干细胞维持培养基和分化培养基，仅在暴露于下部隔室培养基的EB下部实现了局部分化状态。该系统可能使我们能够使用EB重建复杂组织并重现发育过程。
