Jingushi S, Mitsuyama M, Moriya T, Amako K
Infect Immun. 1987 Jul;55(7):1600-6. doi: 10.1128/iai.55.7.1600-1606.1987.
Monoclonal antibodies (MAbs) were raised against the purified fimbriae of Serratia marcescens US46, a strain expressing three morphologically distinct fimbriae. The widths of these fimbriae were 7, 4.5, and 3 nm, respectively. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the purified fimbriae showed three bands with molecular weights of 21,000, 20,000, and 19,000, respectively. This strain had mannose-resistant (MR) hemagglutinating activity and was agglutinated by yeast cells. Therefore, strain US46 appeared to have both MR and mannose-sensitive fimbriae. In the immunoblot analysis, all MAbs reacted with the 20,000-molecular-weight subunit when given a choice of three differently sized subunits. Immunoelectron microscopy showed these MAbs attached to the MR fimbriae with the largest width (7 nm). The antigenic cross-reactivity of fimbriae was examined by an MAb-mediated agglutination test. All MR strains of S. marcescens and some mannose-sensitive strains were agglutinated by the MAbs. The serological homogeneity of MR fimbriae was confirmed by a spot test, using the crude purified fimbriae from several MR strains of S. marcescens. In other gram-negative rods, clinical isolates of Klebsiella spp. with hemagglutinating activity were agglutinated, but clinical isolates of Escherichia coli and Enterobacter spp. were not.
针对粘质沙雷氏菌US46纯化菌毛制备了单克隆抗体，该菌株表达三种形态不同的菌毛。这些菌毛的宽度分别为7、4.5和3纳米。纯化菌毛的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳显示出三条带，分子量分别为21,000、20,000和19,000。该菌株具有抗甘露糖（MR）血凝活性，可被酵母细胞凝集。因此，US46菌株似乎同时具有MR菌毛和甘露糖敏感菌毛。在免疫印迹分析中，当在三种不同大小的亚基中进行选择时，所有单克隆抗体均与分子量为20,000的亚基发生反应。免疫电子显微镜显示这些单克隆抗体附着在宽度最大（7纳米）的MR菌毛上。通过单克隆抗体介导的凝集试验检测菌毛的抗原交叉反应性。所有粘质沙雷氏菌的MR菌株和一些甘露糖敏感菌株均被单克隆抗体凝集。使用来自几种粘质沙雷氏菌MR菌株的粗纯化菌毛，通过斑点试验证实了MR菌毛的血清学同质性。在其他革兰氏阴性杆菌中，具有血凝活性的肺炎克雷伯菌临床分离株被凝集，但大肠杆菌和肠杆菌属的临床分离株未被凝集。
