Mirelman D, Altmann G, Eshdat Y
J Clin Microbiol. 1980 Apr;11(4):328-31. doi: 10.1128/jcm.11.4.328-331.1980.
A total of 393 clinical bacterial isolates were tested for their ability to agglutinate yeast cells of either Saccharomyces cerevisiae or Candida albicans. A positive agglutination of yeasts that could be prevented by methyl alpha-D-mannoside was taken as an indication for the possible presence of a mannose-specific lectin (carbohydrate-binding protein) on the surface of the tested bacteria. Agglutination tests on glass slides showed that 38% of all the isolates tested were positive in their capacity to agglutinate yeasts. Among the various strains tested, all isolates of Serratia marcescens, Proteus morganii, and Citrobacter diversus, as well as 94% of Klebsiella pneumoniae, were positive. On the other hand, only 46% of the Escherichia coli, 48% of the salmonellae, 44% of the Citrobacter freundii, and 71% of the Aeromonas hydrophila isolates were positive. A quantitative determination of the lectin activity done by observing the agglutination of yeasts in microtiter plates showed that S. marcescens isolates were the most avid binders to the yeast, whereas Klebsiella and Citrobacter isolates were the weakest.
共对393株临床分离细菌进行了检测，以评估它们凝集酿酒酵母或白色念珠菌酵母细胞的能力。能被α-D-甘露糖苷抑制的酵母阳性凝集反应被视为受试细菌表面可能存在甘露糖特异性凝集素（碳水化合物结合蛋白）的指标。玻片凝集试验表明，所有受试分离株中有38%具有凝集酵母的能力。在受试的各种菌株中，粘质沙雷氏菌、摩根氏变形杆菌和异型柠檬酸杆菌的所有分离株，以及94%的肺炎克雷伯菌均呈阳性。另一方面，只有46%的大肠杆菌、48%的沙门氏菌、44%的弗氏柠檬酸杆菌和71%的嗜水气单胞菌分离株呈阳性。通过观察微量滴定板中酵母的凝集来进行凝集素活性的定量测定，结果表明粘质沙雷氏菌分离株是与酵母结合最活跃的，而克雷伯菌和柠檬酸杆菌分离株则是最弱的。
