Lemmer K, Howaldt S, Heinrich R, Roder A, Pauli G, Dorner B G, Pauly D, Mielke M, Schwebke I, Grunow R
Centre for Biological Threats and Special Pathogens, Robert Koch Institute, Berlin, Germany.
Applied Infection Control and Hospital Hygiene, Robert Koch Institute, Berlin, Germany.
J Appl Microbiol. 2017 Nov;123(5):1168-1183. doi: 10.1111/jam.13575. Epub 2017 Oct 16.
The work aimed at developing and evaluating practically relevant methods for testing of disinfectants on contaminated personal protective equipment (PPE).
Carriers were prepared from PPE fabrics and contaminated with Bacillus subtilis spores. Peracetic acid (PAA) was applied as a suitable disinfectant. In method 1, the contaminated carrier was submerged in PAA solution; in method 2, the contaminated area was covered with PAA; and in method 3, PAA, preferentially combined with a surfactant, was dispersed as a thin layer. In each method, 0·5-1% PAA reduced the viability of spores by a factor of ≥6 log within 3 min. The technique of the most realistic method 3 proved to be effective at low temperatures and also with a high organic load. Vaccinia virus and Adenovirus were inactivated with 0·05-0·1% PAA by up to ≥6 log within 1 min. The cytotoxicity of ricin was considerably reduced by 2% PAA within 15 min of exposure.
PAA/detergent mixture enabled to cover hydrophobic PPE surfaces with a thin and yet effective disinfectant layer.
The test methods are objective tools for estimating the biocidal efficacy of disinfectants on hydrophobic flexible surfaces.
本研究旨在开发和评估针对受污染个人防护装备（PPE）进行消毒剂测试的实际相关方法。
用PPE织物制备载体并接种枯草芽孢杆菌孢子。使用过氧乙酸（PAA）作为合适的消毒剂。在方法1中，将受污染的载体浸入PAA溶液中；在方法2中，用PAA覆盖受污染区域；在方法3中，将PAA（优先与表面活性剂混合）分散成薄层。在每种方法中，0.5 - 1%的PAA在3分钟内可使孢子活力降低≥6个对数级。最符合实际情况的方法3在低温以及高有机负荷条件下均被证明有效。痘苗病毒和腺病毒在1分钟内用0.05 - 0.1%的PAA灭活达≥6个对数级。蓖麻毒素的细胞毒性在暴露于2%的PAA 15分钟内显著降低。
PAA/洗涤剂混合物能够在疏水性PPE表面覆盖一层薄而有效的消毒层。
这些测试方法是评估消毒剂对疏水性柔性表面杀菌效果的客观工具。
