活细胞中的离子强度传感
Ionic Strength Sensing in Living Cells.
作者信息
Liu Boqun, Poolman Bert, Boersma Arnold J
机构信息
Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute & Zernike Institute for Advanced Materials, University of Groningen , Nijenborgh 4, 9747 AG Groningen, The Netherlands.
出版信息
ACS Chem Biol. 2017 Oct 20;12(10):2510-2514. doi: 10.1021/acschembio.7b00348. Epub 2017 Sep 6.
Knowledge of the ionic strength in cells is required to understand the in vivo biochemistry of the charged biomacromolecules. Here, we present the first sensors to determine the ionic strength in living cells, by designing protein probes based on Förster resonance energy transfer (FRET). These probes allow observation of spatiotemporal changes in the ionic strength on the single-cell level.
要理解带电生物大分子的体内生物化学过程，就需要了解细胞中的离子强度。在此，我们通过设计基于荧光共振能量转移（FRET）的蛋白质探针，展示了首批用于测定活细胞中离子强度的传感器。这些探针能够在单细胞水平上观察离子强度的时空变化。
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