Tóth Ákos, Baka Erzsébet, Bata-Vidács Ildikó, Luzics Szabina, Kosztik Judit, Tóth Erika, Kéki Zsuzsa, Schumann Peter, Táncsics András, Nagy István, Sós Endre, Kukolya József
Department of Applied and Environmental Microbiology, Agro-Environmental Research Institute, National Agricultural Research and Innovation Centre, H-1022 Budapest, Herman Ottó u. 15, Hungary.
Department of Microbiology, Eötvös Loránd University, H-1117 Budapest, Pázmány P. stny. 1/C, Hungary.
Int J Syst Evol Microbiol. 2017 Aug;67(8):2758-2765. doi: 10.1099/ijsem.0.002018.
A Gram-stain-positive bacterium, designated TSL3T, was isolated from faeces of a porcupine, Hystrix indica, from the Budapest Zoo and Botanical Garden, Hungary. On the basis of 16S rRNA gene sequence analysis, the strain is phylogenetically related to the family Micrococcaceae. The highest 16S rRNA gene sequence similarity was found with Micrococcus terreus V3M1T (96.50 %) followed by Arthrobacter humicola KV-653T (96.43 %). Cells of strain TSL3T were aerobic, non-motile and coccoid-shaped. The main fatty acids were anteiso-C15 : 0 (54.4 %), iso-C16 : 0 (18.2 %) and iso C15 : 0 (9.7 %). The major menaquinone was MK-7, and the polar lipid profile included phosphatidylglycerol, diphosphatidylglycerol, dimannosylglyceride, trimannosyldiacylglycerol, phosphatidylinositol, three unknown phospholipids and two unknown glycolipids. Strain TSL3T showed the peptidoglycan structure A4alpha l-Lys - Gly - l-Glu. The DNA G+C content of strain TSL3T was 58.4 mol%. Phenotypic and genotypic characterisation clearly showed that strain TSL3T could be differerentiated from the members of other genera in the family Micrococcaceae. According to these results, strain TSL3T represents a novel genus and species, for which the name Micrococcoides hystricis gen. nov., sp. nov. is proposed. The type strain is TSL3T (=DSM 29785T=NCAIM B. 02604T).
从匈牙利布达佩斯动物园和植物园的印度豪猪粪便中分离出一株革兰氏阳性菌，命名为TSL3T。基于16S rRNA基因序列分析，该菌株在系统发育上与微球菌科相关。发现其16S rRNA基因序列与土生微球菌V3M1T的相似度最高（96.50%），其次是栖土节杆菌KV-653T（96.43%）。TSL3T菌株的细胞为需氧型、不运动且呈球状。主要脂肪酸为anteiso-C15 : 0（54.4%）、iso-C16 : 0（18.2%）和iso C15 : 0（9.7%）。主要甲基萘醌为MK-7，极性脂质谱包括磷脂酰甘油、二磷脂酰甘油、二甘露糖基甘油酯、三甘露糖基二酰基甘油、磷脂酰肌醇、三种未知磷脂和两种未知糖脂。TSL3T菌株显示出肽聚糖结构A4alpha l-Lys - Gly - l-Glu。TSL3T菌株的DNA G+C含量为58.4 mol%。表型和基因型特征清楚地表明，TSL3T菌株可与微球菌科其他属的成员区分开来。根据这些结果，TSL菌株代表一个新的属和种，为此提出新属名为Micrococcoides hystricis gen. nov.，新种名为Micrococcoides hystricis sp. nov.。模式菌株为TSL3T（=DSM 29785T=NCAIM B. 02604T）。
