Nakamura T, Yanagihara R, Gibbs C J, Gajdusek D C
J Infect Dis. 1985 Apr;151(4):691-7. doi: 10.1093/infdis/151.4.691.
To clarify the basis of the age-dependent susceptibility of infant mice to fatal meningoencephalitis caused by Hantaan virus, we investigated the ability of spleen cells from immune and nonimmune weanling BALB/c mice to confer protection to syngeneic infant mice. Intraperitoneal transfer of 10 X 10(7), 5 X 10(7), 2.5 X 10(7), and 1.2 X 10(7) immune spleen cells to infant mice 24 hr after intracerebral challenge with 100 50% lethal doses of Hantaan virus (strain 76-118) resulted in 100%, 70%, 64%, and 30% protection, respectively. Even as late as 48 hr after virus challenge, transfer of 10 X 10(7) immune spleen cells conferred complete protection. In contrast, nonimmune spleen cells offered no protection, even when cells were transferred 48 hr before virus challenge. The protective capacity of immune spleen cells was abolished following treatment with antibody to theta antigen and guinea pig complement, but was preserved after the depletion of B cells, a result suggesting that T cells play a crucial role in the resistance of mice to fatal Hantaan virus infection.
为阐明幼鼠对汉坦病毒所致致命性脑膜脑炎年龄依赖性易感性的基础，我们研究了免疫和未免疫的断奶BALB/c小鼠脾细胞赋予同基因幼鼠保护的能力。在用100个50%致死剂量的汉坦病毒（76-118株）脑内攻击幼鼠24小时后，向其腹腔注射10×10⁷、5×10⁷、2.5×10⁷和1.2×10⁷个免疫脾细胞，分别产生了100%、70%、64%和30%的保护率。即使在病毒攻击后48小时，注射10×10⁷个免疫脾细胞仍能提供完全保护。相比之下，未免疫的脾细胞即使在病毒攻击前48小时注射也不能提供保护。用抗θ抗原抗体和豚鼠补体处理后，免疫脾细胞的保护能力丧失，但在B细胞耗竭后仍保留，这一结果表明T细胞在小鼠抵抗致命性汉坦病毒感染中起关键作用。
