C&K genomics, C-1008, H businesspark, 26, Beobwon-ro 9-gil, Songpa-gu, Seoul, Republic of Korea.
Interdisciplinary Program in Bioinformatics, Seoul National University, Seoul, Republic of Korea.
Sci Rep. 2017 Aug 30;7(1):10048. doi: 10.1038/s41598-017-10106-6.
The tail of many animal species is responsible for various physiological functions. The functional importance of tail may have brought tail-loss to attention in many evolutionary and developmental studies. To provide a better explanation for the loss of tail, the current study aims to identify the evolutionary history and putative causal variants for the short tail in DongGyeongi (DG), an endangered dog breed, which is also the only dog in Korea that possesses a short tail. Whole genome sequencing was conducted on 22 samples of DG, followed by an investigation of population stratification with 10 other dog breeds. The genotypes, selective sweep and demography of DG were also investigated. As a result, we discovered the unique genetic structure of DG and suggested two possible ways in which the short tail phenotype developed. Moreover, this study suggested that selective sweep genes, ANKRD11 and ACVR2B may contribute to the reduction in tail length, and non-synonymous variant in the coding sequence of T gene and the CpG island variant of SFRP2 gene are the candidate causal variants for the tail-loss.
许多动物物种的尾巴负责各种生理功能。尾巴的功能重要性可能使尾巴的缺失在许多进化和发育研究中受到关注。为了更好地解释尾巴的缺失，本研究旨在确定 DongGyeongi（DG）短尾的进化历史和潜在的因果变异，DongGyeongi 是一种濒危犬种，也是韩国唯一拥有短尾的犬种。对 22 个 DG 样本进行了全基因组测序，然后对 10 个其他犬种进行了群体分层调查。还研究了 DG 的基因型、选择清扫和人口动态。结果发现了 DG 独特的遗传结构，并提出了两种可能的短尾表型发育方式。此外，这项研究表明，选择性清扫基因 ANKRD11 和 ACVR2B 可能导致尾巴长度的减少，而 T 基因编码序列中的非同义变异和 SFRP2 基因的 CpG 岛变异是尾巴缺失的候选因果变异。
