Alwhibi Mona Soliman
Department of Botany and Microbiology, King Saud University, P.O. Box: 22452, Riyadh 11495, Saudi Arabia.
Saudi J Biol Sci. 2017 Sep;24(6):1404-1409. doi: 10.1016/j.sjbs.2016.08.008. Epub 2016 Aug 30.
, is a shrub that has ecological importance. The height of the plant differs from one population to another and the difference in length of the inflorescence can be attributed to environmental factors, such as rainfall or type of soil and temperature. To date, no study has shed light on estimation in seed samples of in Saudi Arabia. So, the aim is to evaluate and characterize the protein patterns of seed storage proteins of to be used as fingerprint of this plant in Saudi Arabia. It is collected from different locations in the central region of Saudi Arabia and total protein extraction from plant was compared in SDS-PAGE. The genetic relationships among all cultivars were analyzed using UPGMA and NJ using Total Lab TL and in the same way using Jaccard Similarity Coefficient dendrogram using STATISTICA (ver.8) software. Results, our data show that amounts of protein are different, although they are of the same type or from the same geographical region. Amounts ranged between 22 and 1.5 mg/g of dry weight. Less amount of protein was obtained from the group of samples collected from Dir'iyah area, and the highest amount of protein was from the group of samples collected from Dyrab area in general.
[植物名称]是一种具有生态重要性的灌木。该植物的高度因种群而异，花序长度的差异可归因于环境因素，如降雨量、土壤类型和温度。迄今为止，尚未有关于沙特阿拉伯[植物名称]种子样本估计的研究。因此，目的是评估和表征[植物名称]种子贮藏蛋白的蛋白质模式，以用作该植物在沙特阿拉伯的指纹图谱。它从沙特阿拉伯中部地区的不同地点收集，并在SDS - PAGE中比较了从植物中提取的总蛋白。使用Total Lab TL通过UPGMA和NJ分析所有品种之间的遗传关系，并使用STATISTICA（版本8）软件以相同方式使用杰卡德相似系数树状图进行分析。结果，我们的数据表明，尽管蛋白质类型相同或来自同一地理区域，但蛋白质含量不同。含量范围在每克干重22至1.5毫克之间。从迪里亚地区收集的样本组中获得的蛋白质含量较少，总体而言，从迪拉布地区收集的样本组中蛋白质含量最高。
