Alawhibi Mona S, AlSubeie Moodi S, Bukhari Najat A, Soliman Dina A
King Saud University, Saudi Arabia.
Imam Muhammad bin Saud Islamic University, Saudi Arabia.
Saudi J Biol Sci. 2020 Jul;27(7):1824-1829. doi: 10.1016/j.sjbs.2020.04.048. Epub 2020 May 7.
The purpose of write this paper is to study the genetic variability between and within different populations in different regions of Saudi arabia Kingdom, using the determination of genetic fingerprint method by Inter Simple Sequence Repeat (ISSR). Because this plant highly vulnerable to depletion by humans in all places of existence, it is an economically valuable plant where raft is an important pastoral resource in central and northern Arabia. It also has multiple medical uses. It is a plant that can withstand abiotic stresses such as drought and high temperature, making it suitable for cultivation in marginal lands in arid areas. All of the above was a catalyst for plant characterization using a number of samples, collected from different regions of Saudi Arabia to find out the genetic variation of species, genetic diversity in knowing the plant community is an essential step towards the design of programs for plant breeding as well as preserved from extinction. This was done using the Inter Simple Sequence Repeat (ISSR). The results showed there were significant differences and molecular differences between plant samples. The average polymorphism between the genetic inputs of the studied samples was 53.7%, and this percentage of genetic variability is significant for progress in growth and plant regeneration in the face of unfair practices against it, in addition to adverse environmental conditions in most years. As evidenced by the percentage of matrix similarity. The ISSR results indicate that the genotype between five different regions genotypes ranged from 0.365 to 0.527, indicating that is a local plant capable of surviving and adapting to the environmental conditions in Saudi Arabia through the positive change in the genetic makeup of this species.
撰写本文的目的是利用简单序列重复区间（ISSR）基因指纹测定方法，研究沙特阿拉伯王国不同地区不同种群之间以及种群内部的遗传变异性。由于这种植物在其所有生存地都极易因人类活动而枯竭，它是一种具有经济价值的植物，在阿拉伯半岛中部和北部，筏草是一种重要的牧草资源。它还具有多种药用价值。它是一种能够耐受干旱和高温等非生物胁迫的植物，使其适合在干旱地区的边际土地上种植。上述所有因素促使我们使用从沙特阿拉伯不同地区采集的多个样本对该植物进行特征分析，以找出物种的遗传变异，了解植物群落中的遗传多样性是设计植物育种计划以及防止植物灭绝的重要一步。这是通过简单序列重复区间（ISSR）来完成的。结果表明，植物样本之间存在显著差异和分子差异。所研究样本的遗传输入之间的平均多态性为53.7%，这一遗传变异性百分比对于该植物在面对针对它的不当行为以及大多数年份的不利环境条件时的生长和再生进展具有重要意义。矩阵相似性百分比证明了这一点。ISSR结果表明，五个不同地区基因型之间的基因型相似性范围为0.365至0.527，表明该植物是一种能够通过该物种基因组成的积极变化在沙特阿拉伯的环境条件下生存和适应的本地植物。
