氯化铝通过抑制大鼠 ATP 酶活性和促性腺激素受体表达导致睾丸功能障碍。
Aluminum Chloride Causes the Dysfunction of Testes Through Inhibiting the ATPase Enzyme Activities and Gonadotropin Receptor Expression in Rats.
机构信息
Key Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and Treatment, Northeast Agricultural University, Harbin, 150030, China.
Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun, Jilin, 130062, China.
出版信息
Biol Trace Elem Res. 2018 Jun;183(2):296-304. doi: 10.1007/s12011-017-1120-1. Epub 2017 Aug 30.
The aim of this experiment is to explore the effects of aluminum chloride (AlCl) on the ATPase enzymes and gonadotropin receptors in the testes. Eighty male Wistar rats were orally exposed to 0 mg/kg body weight (BW) (control group, CG), 64 mg/kg BW (low-dose group, LG), 128 mg/kg BW (mid-dose group, MG), or 256 mg/kg BW (high-dose group, HG) for 120 days. The microstructure and ultrastructure of testes; the activities of Na-K-ATPase, Mg-ATPase, and Ca-ATPase; and the mRNA and protein expressions of follicle-stimulating hormone receptor (FSHR) and luteinizing hormone receptors (LHR) in the testes were examined. The results showed that the testes histological structure were damaged; the activities of Na-K-ATPase, Mg-ATPase, and Ca-ATPase, the mRNA and protein expressions of FSHR and LHR in the testes were all decreased in the rats with AlCl exposure. It indicates that AlCl causes the dysfunction of testes in rats.
本实验旨在探讨氯化铝（AlCl）对睾丸中 ATP 酶和促性腺激素受体的影响。将 80 只雄性 Wistar 大鼠经口暴露于 0mg/kg 体重（BW）（对照组，CG）、64mg/kg BW（低剂量组，LG）、128mg/kg BW（中剂量组，MG）或 256mg/kg BW（高剂量组，HG）120 天。检测睾丸的微观结构和超微结构；Na-K-ATP 酶、Mg-ATP 酶和 Ca-ATP 酶的活性；以及睾丸中促卵泡激素受体（FSHR）和黄体生成素受体（LHR）的 mRNA 和蛋白表达。结果表明，AlCl 暴露组大鼠睾丸组织结构受损；睾丸中 Na-K-ATP 酶、Mg-ATP 酶和 Ca-ATP 酶的活性、FSHR 和 LHR 的 mRNA 和蛋白表达均降低。这表明 AlCl 导致大鼠睾丸功能障碍。
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