铝暴露对雄性大鼠血清性激素和雄激素受体表达的影响。
Effects of aluminum exposure on serum sex hormones and androgen receptor expression in male rats.
机构信息
College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China.
出版信息
Biol Trace Elem Res. 2011 Dec;144(1-3):1050-8. doi: 10.1007/s12011-011-9098-6. Epub 2011 Jun 7.
The effects of aluminum (Al) exposure on reproductive functions of male rats were investigated. Forty male Wistar rats (4 weeks old) weighing 75-95 g were randomly divided into four groups and orally exposed to 0 (control group GC), 64.18 (low-dose group GL), 128.36 (middle-dose group GM), and 256.72 (high-dose group GH) mg/kg aluminum trichloride in drinking water for 120 days. The levels of testosterone (T), follicle-stimulating hormone (FSH), and luteinizing hormone (LH) were determined by radioimmunoassay. The androgen receptor (AR) expressions in testes were detected respectively by immunohistochemistry and time quantitative PCR. Results showed that the levels of T and LH in GM and GH were lower than those in GC (P < 0.05), but there were no significant changes in FSH level in all Al-treated groups (P > 0.05). AR protein expressions in GM and GH were lower than those in GC (P < 0.05), and there was a dose-response relationship between Al-exposure doses and AR protein expressions. The levels of AR mRNA expressions were lower in all Al-treated groups than those of GC (P < 0.05). The results indicate that Al can cause endocrinal disorders and interfere with AR expression, which suppresses development and functional maintenance of the testes.
研究了铝（Al）暴露对雄性大鼠生殖功能的影响。将 40 只雄性 Wistar 大鼠（4 周龄），体重 75-95g，随机分为 4 组，分别经口给予 0（对照组 GC）、64.18（低剂量组 GL）、128.36（中剂量组 GM）和 256.72（高剂量组 GH）mg/kg 三氯化铝饮用水，染毒 120 天。采用放射免疫法测定睾酮（T）、卵泡刺激素（FSH）和黄体生成素（LH）水平。分别采用免疫组织化学法和实时定量 PCR 法检测睾丸雄激素受体（AR）的表达。结果显示，GM 和 GH 组 T 和 LH 水平均低于 GC 组（P<0.05），但各染铝组 FSH 水平无明显变化（P>0.05）。GM 和 GH 组 AR 蛋白表达均低于 GC 组（P<0.05），且 AR 蛋白表达与 Al 暴露剂量呈剂量-反应关系。各染铝组 AR mRNA 表达水平均低于 GC 组（P<0.05）。结果表明，Al 可导致内分泌紊乱，干扰 AR 表达，抑制睾丸发育和功能维持。
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