Mago Rohit, Till Bradley, Periyannan Sambasivam, Yu Guotai, Wulff Brande B H, Lagudah Evans
Agriculture & Food, CSIRO, Canberra, ACT, Australia.
Plant Breeding and Genetics Laboratory, International Atomic Energy Agency, Vienna, Austria.
Methods Mol Biol. 2017;1659:199-205. doi: 10.1007/978-1-4939-7249-4_17.
One of the most important tools to identify and validate rust resistance gene function is by producing loss-of-function mutants. Mutants can be produced using irradiation, chemicals, and insertions. Among all the mutagens, ethyl methanesulfonate (EMS) and sodium azide are most favored because of the ease of use and generation of random point mutations in the genome. The mutants so produced facilitate the isolation, identification and cloning of rust resistance genes. In this chapter we describe a protocol for seed mutagenesis of wheat with EMS and sodium azide.
鉴定和验证抗锈病基因功能的最重要工具之一是产生功能丧失型突变体。突变体可以通过辐射、化学物质和插入来产生。在所有诱变剂中，甲磺酸乙酯（EMS）和叠氮化钠最受青睐，因为它们使用方便且能在基因组中产生随机点突变。如此产生的突变体有助于抗锈病基因的分离、鉴定和克隆。在本章中，我们描述了一种用EMS和叠氮化钠对小麦种子进行诱变的方案。
