Sharma Raghvendra, Chen Chunhong, Zhang Peng, Bharti Hemlata, Vikas Venu Kumaran, Norman Michael, Dibley Katherine, Riaz Adnan, Hewitt Tim, Hoxha Sami, Forrest Kerrie, Lagudah Evans, Bariana Harbans, Bansal Urmil, Hickey Lee, Periyannan Sambasivam
Commonwealth Scientific and Industrial Research Organization Agriculture and Food, Canberra, ACT, 2601, Australia.
Centre for Crop Science, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Brisbane, QLD, 4072, Australia.
Theor Appl Genet. 2025 Jul 9;138(8):180. doi: 10.1007/s00122-025-04965-1.
Comparative genomic analysis of two all-stage stripe rust resistance loci from Vavilov wheat landrace accession, AGG40807WHEA1, using Chinese Spring and 10 + hexaploid wheat genomes and validation of closely linked KASP markers. The ongoing occurrence and spread of wheat stripe rust, caused by the fungal pathogen Puccinia striiformis f. sp. tritici, threatens the global food security. Cultivation of varieties with effective sources of resistance is often followed by the appearance of virulent pathotypes at various times after their introduction. This requires an ongoing search for new sources. Tests of 296 accessions from the Vavilov wheat landrace collection identified numerous lines with broadly effective all-stage stripe rust resistance. Genetic analysis of one of these accessions (Australian Grains Genebank number AGG40807WHEA1) identified two all-stage resistance genes, temporarily named YrV1 and YrV2. The YRV1 and YRV2 loci were mapped to 3.48-3.98 and 730.2-731.2 Mb intervals in the short arm of chromosome 3B and the long arm of chromosome 7B, respectively. A comparative genomic analysis of the YRV1 locus in the Chinese Spring and the 10 + wheat pangenome databases revealed genomic rearrangements and lack of sequences encoding a nucleotide-binding and leucine-rich repeat (NLR) domain protein. Sequences belonging to NLR-like genes were present in the YRV2 region. Kompetitive allele-specific PCR (KASP) markers designed from SNPs IWB71814 and IWB69562, located at 0.4 cM and 0.5 cM distal to YrV1 and YrV2, respectively, were validated for marker-assisted selection using 123 hexaploid and 15 tetraploid wheat and 14 triticale cultivars. YrV1 and YrV2 genes are potentially valuable resources, and use of the closely linked molecular markers will expedite their deployment in breeding.
利用中国春小麦和10+六倍体小麦基因组对来自瓦维洛夫小麦地方品种AGG40807WHEA1的两个全生育期条锈病抗性位点进行比较基因组分析，并验证紧密连锁的KASP标记。由真菌病原体条形柄锈菌小麦专化型引起的小麦条锈病持续发生和传播，威胁着全球粮食安全。种植具有有效抗性来源的品种后，往往会在引入后的不同时间出现毒性致病型。这就需要不断寻找新的抗性来源。对瓦维洛夫小麦地方品种收集的296份材料进行测试，鉴定出许多具有广泛有效全生育期条锈病抗性的品系。对其中一份材料（澳大利亚谷物基因库编号AGG40807WHEA1）进行遗传分析，鉴定出两个全生育期抗性基因，暂时命名为YrV1和YrV2。YRV1和YRV2位点分别定位在3B染色体短臂的3.48-3.98 Mb区间和7B染色体长臂的730.2-731.2 Mb区间。对中国春小麦和10+小麦泛基因组数据库中YRV1位点的比较基因组分析揭示了基因组重排以及缺乏编码核苷酸结合和富含亮氨酸重复（NLR）结构域蛋白的序列。YRV2区域存在属于NLR样基因的序列。从分别位于YrV1和YrV2远端0.4 cM和0.5 cM的单核苷酸多态性IWB71814和IWB69562设计的竞争性等位基因特异性PCR（KASP）标记，使用123个六倍体和15个四倍体小麦以及14个小黑麦品种进行标记辅助选择验证。YrV1和YrV2基因是潜在的宝贵资源，使用紧密连锁的分子标记将加快它们在育种中的应用。
