Department of Anesthesiology and Reanimation, Konya Training and Research Hospital, Konya, Turkey.
Department of Psychiatry, Konya Training and Research Hospital, Konya, Turkey.
Am J Ind Med. 2017 Nov;60(11):1003-1009. doi: 10.1002/ajim.22764. Epub 2017 Aug 30.
The purpose of this study was to investigate the association between dynamic thiol/disulphide homeostasis and occupational exposure to volatile anesthetic gases in operating theater personnel. Decreased blood thiol levels and raised blood disulphide levels serve as biomarkers of oxidative stress.
We included 65 subjects occupationally exposed and 55 unexposed healthy medical professionals into the study. A novel method enabled separate measurements of components involved in dynamic thiol/disulphide homeostasis (native thiol, disulphide, and total thiol). To control for the potential confounding effect on oxidative stress of psychological symptoms potentially caused by occupational stress, we used scores obtained from four different anxiety and depression inventories.
Mean ± standard deviation native thiol was found to be 433.35 ± 30.68 in the exposed group, lower than among controls, 446.61 ± 27.8 (P = 0.02). Disulphide in the exposed group was 15.78 ± 5.12, higher than among controls, 12.14 ± 5.33 (P < 0.001). After adjusting for anxiety and depression scores, age and gender, native thiol remained lower and disulphide higher in the exposed group (P = 0.008 and P < 0.001).
Dynamic thiol/disulphide homeostasis in workers exposed to anesthetic gases was found to be disturbed after adjusting for the possible contribution of anxiety. We infer that this is due to the oxidative effect of exposure to anesthetic gases.
本研究旨在探讨手术室工作人员职业性接触挥发性麻醉气体与动态硫醇/二硫键动态平衡之间的关系。血液硫醇水平降低和二硫键水平升高可作为氧化应激的生物标志物。
我们将 65 名职业接触者和 55 名未接触者纳入研究。一种新方法可分别测量动态硫醇/二硫键动态平衡（天然硫醇、二硫键和总硫醇）的组成部分。为了控制职业应激引起的心理症状对氧化应激的潜在混杂影响，我们使用了来自四个不同焦虑和抑郁量表的得分。
暴露组的平均±标准差天然硫醇为 433.35±30.68，低于对照组的 446.61±27.8（P=0.02）。暴露组的二硫键为 15.78±5.12，高于对照组的 12.14±5.33（P<0.001）。在调整焦虑和抑郁评分、年龄和性别后，暴露组的天然硫醇仍较低，二硫键仍较高（P=0.008 和 P<0.001）。
在调整焦虑可能的影响后，我们发现接触麻醉气体的工人的动态硫醇/二硫键动态平衡受到干扰。我们推断这是由于接触麻醉气体的氧化作用。
