Gündüzöz Meşide, Birgin İritaş Servet, Tutkun Lütfiye, Büyükşekerci Murat, Pinar Çetintepe Sultan, Bal Ceylan, Alişik Murat, Erdoğan Serpil, Yilmaz Hinç, Erel Özcan
Department of Family Medicine, Occupational Diseases Hospital, Ankara, Turkey.
Council of Forensic Medicine, Branch Office of Ankara, Ankara, Turkey.
Cent Eur J Public Health. 2018 Sep;26(3):190-194. doi: 10.21101/cejph.a4972.
The aim of this study was to investigate the associations between urinary arsenic, oxidative stress, assessed by thiol/disulphide homeostasis, and lung diseases in firefighters.
The study conducted among the municipality-based male firefighters (n = 100) who were admitted to occupational diseases clinic for periodic medical examination. The control group consisted of non-exposed male office workers (n = 50). Urinary arsenic levels, thiol/disulphide homeostasis parameters of participants were determined. Also, lung diseases were assessed by chest X-ray and pulmonary function tests.
The mean age and work year did not differ in the study and control group. The median urinary arsenic concentration of firefighters was significantly higher than in the control group: 15.65 (2.5-246) μg/L and 3 (0.10-6) μg/L, respectively (p < 0.001). The parameters of pulmonary function tests (PFT) FVC (%), FEV1 (%), FEV1/FVC ratio and FEF 25-75 (%) were all significantly lower in firefighters compared to controls. A significant increase in mean serum disulphide concentration (17.10 ± 8.31 μmol/L vs. 7.48 ± 5.91) (Fig. 1) and disulphide/native thiol % ratio: 3.63 (0.53-11.43) vs. 1.51 (0.03-7.65) (p < 0.001) were found between exposed group and controls. The Spearman's correlation analysis revealed a positive correlation between urinary arsenic and disulphide (r = 0.422, p < 0.001), disulphide/native thiol % ratio (r = 0.409, p < 0.001). Nevertheless, urinary arsenic correlated negatively with all PFT parameters including FVC (%), FEV1 (%), FEV1/FVC and FEF 25-75 (%) (p < 0.001).
We showed the arsenic-induced oxidative stress in firefighters with impairments of several lung functions determined by thiol/disulphide homeostasis using a novel method.
本研究旨在调查消防员尿砷、通过硫醇/二硫化物稳态评估的氧化应激与肺部疾病之间的关联。
该研究在以市为基础的男性消防员（n = 100）中进行，这些消防员因定期体检入住职业病诊所。对照组由未接触的男性办公室职员（n = 50）组成。测定了参与者的尿砷水平、硫醇/二硫化物稳态参数。此外，通过胸部X线和肺功能测试评估肺部疾病。
研究组和对照组的平均年龄和工作年限无差异。消防员的尿砷浓度中位数显著高于对照组：分别为15.65（2.5 - 246）μg/L和3（0.10 - 6）μg/L（p < 0.001）。与对照组相比，消防员的肺功能测试（PFT）参数FVC（%）、FEV1（%）、FEV1/FVC比值和FEF 25 - 75（%）均显著降低。暴露组与对照组之间血清二硫化物平均浓度显著升高（17.10 ± 8.31 μmol/L对7.48 ± 5.91）（图1）以及二硫化物/天然硫醇%比值：3.63（0.53 - 11.43）对1.51（0.03 - 7.65）（p < 0.001）。Spearman相关性分析显示尿砷与二硫化物（r = 0.422，p < 0.001）、二硫化物/天然硫醇%比值（r = 0.409，p < 0.001）呈正相关。然而，尿砷与所有PFT参数包括FVC（%）、FEV1（%）、FEV1/FVC和FEF 25 - 75（%）呈负相关（p < 0.001）。
我们使用一种新方法表明，消防员中存在砷诱导的氧化应激，伴有通过硫醇/二硫化物稳态确定的多种肺功能受损。
