Division of Pedodontics, Malatya Oral and Dental Heath Hospital, Republic of Turkey Ministry of Health, Malatya, Turkey.
Department of Pedodontics, Faculty of Dentistry, Mustafa Kemal University, Hatay, Turkey.
Eur J Paediatr Dent. 2020 Mar;21(1):13-17. doi: 10.23804/ejpd.2020.21.01.03.
Oral streptococci were found to be associated with Early Childhood Caries. The aim of this study was to evaluate the 6 different bacteria in the streptococcus group in the saliva of children with severe early childhood caries (S-ECC) by polymerase chain reaction (PCR).
A total of 60 children between 3 and 6 years of age were divided into two groups: children with S-ECC (Group S-ECC; n=30) and children who were caries-free (Group CF; n=30), according to the dmft and dmfs indices. Unstimulated saliva was collected from all participants for the detection of streptococcal group bacteria, including: Streptococcus mutans, Streptococcus oralis, Streptococcus sanguinis, Streptococcus gordonii, Streptococcus salivarius, and Streptococcus sobrinus, using PCR-restriction fragment length polymorphism (RFLP) of amplified 16S rRNA gene. The data were analysed using SPSS software.
The prevalence of S. oralis was significantly higher in the S-ECC group compared to the CF group (p<0.05). However, the frequencies of S. mutans, S. sanguinis, S. gordonii, S. salivarius, and S. sobrinus were similar between the two groups (p>0.05). The amount of streptococci colonies was higher in the S-ECC group compared to the CF group (p<0.05).
S. mutans or S. sobrinus alone may not be the only indicators for high risk of caries, but the prevalence of S. oralis in saliva may be a risk factor for increased caries activity in S-ECC.
口腔链球菌与幼儿龋病有关。本研究旨在通过聚合酶链反应（PCR）评估唾液中严重幼儿龋（S-ECC）儿童中链球菌属的 6 种不同细菌。
根据 dmft 和 dmfs 指数，将 60 名 3 至 6 岁儿童分为两组：患有 S-ECC 的儿童（S-ECC 组，n=30）和无龋儿童（CF 组，n=30）。所有参与者均采集未刺激唾液，用于检测链球菌属细菌，包括：变形链球菌、口腔链球菌、血链球菌、戈登链球菌、唾液链球菌和咽峡链球菌，使用扩增 16S rRNA 基因的 PCR-限制性片段长度多态性（RFLP）。使用 SPSS 软件分析数据。
与 CF 组相比，S-ECC 组中 S. oralis 的检出率显著更高（p<0.05）。然而，两组中 S. mutans、S. sanguinis、S. gordonii、S. salivarius 和 S. sobrinus 的频率相似（p>0.05）。S-ECC 组的链球菌菌落数量高于 CF 组（p<0.05）。
单独的 S. mutans 或 S. sobrinus 可能不是高龋风险的唯一指标，但唾液中 S. oralis 的流行可能是 S-ECC 龋病活动增加的危险因素。
