Departamento de Medio Ambiente, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Ctra de la Coruña, 7, 28040 Madrid, Spain.
Departamento de Medio Ambiente, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Ctra de la Coruña, 7, 28040 Madrid, Spain.
J Pharm Biomed Anal. 2017 Nov 30;146:79-85. doi: 10.1016/j.jpba.2017.08.013. Epub 2017 Aug 12.
Macrolides are one of the most commonly used families of antibiotics employed in human and veterinary treatment. These compounds are considered emerging contaminants with potential ecological and human health risks that could be present in surface water. This paper describes the development and application of a simple and efficient extraction procedure for the determination of tilmicosin; erythromycin, tylosin and erythromycin-HO from water samples. Sample extraction was carried out using magnetic solid-phase extraction using oleate functionalized magnetic nanoparticles followed by LC-MS/MS analysis. The effects of several parameters on the extraction efficiency of MLs from water were evaluated. The recovery results obtained were >84% for most of the compounds, except for erytromycin. The LOD and LOQ values ranged from 11.5 to 26ngL and from 34 to 77ngL, respectively. The selected method was applied to monitor these contaminants in water samples from different sources. Tilmicosin and tylosin were not detected in any of the samples, but erythromycin and erythromycin-HO were found in 50% of the surface water samples at levels from <LOQ to 264ngL and 149ngL, respectively.
大环内酯类抗生素是人类和兽医治疗中最常用的抗生素家族之一。这些化合物被认为是具有潜在生态和人类健康风险的新兴污染物，可能存在于地表水。本文描述了一种简单有效的提取方法，用于从水样中测定替米考星；红霉素、泰乐菌素和红霉素-HO。采用油酸功能化磁性纳米粒子进行磁固相萃取，对水样进行了萃取，然后进行 LC-MS/MS 分析。考察了多种参数对 MLs 从水中萃取效率的影响。除红霉素外，大多数化合物的回收率均大于 84%。LOD 和 LOQ 值分别为 11.5 至 26ngL 和 34 至 77ngL。该方法用于监测不同来源水样中的这些污染物。在任何水样中均未检测到替米考星和泰乐菌素，但在 50%的地表水样品中检测到红霉素和红霉素-HO，浓度分别为<LOQ 至 264ngL 和 149ngL。
