de Jong A J, Klamer M, Jansen J B, Lamers C B
Regul Pept. 1987 May;17(5):285-93. doi: 10.1016/0167-0115(87)90286-2.
Infusion of the neuropeptide bombesin stimulates the secretion of several gastrointestinal hormones by an unknown mechanism. We have investigated the effects of atropine (15 ng/kg as bolus followed by 2.5 ng/kg X 30 min) and somatostatin (125 micrograms as i.v. bolus followed by 62.5 micrograms/30 min) on the stimulation of 3 hormones (gastrin, cholecystokinin and pancreatic polypeptide) by 60 pmol/kg X 20 min bombesin in 6 healthy volunteers. Plasma samples for measurement of hormones by sensitive and specific radioimmunoassays were obtained at -5, 0, 2.5, 5, 7.5, 10, 15, 20, 25 and 30 min. Bombesin induced significant increases in plasma gastrin (12 +/- 2 to 34 +/- 3 pM; P less than 0.0005), cholecystokinin (1.2 +/- 0.2 to 8.9 +/- 0.7 pM; P less than 0.0001) and pancreatic polypeptide (22 +/- 4 to 72 +/- 19 pM; P less than 0.05). There were great differences between the effects of atropine and somatostatin on the hormonal responses to bombesin. Atropine slightly increased the response of gastrin by 19% and that of cholecystokinin by 15%, but strongly inhibited the bombesin-stimulated pancreatic polypeptide secretion by 97%. On the other hand, somatostatin inhibited the bombesin-induced secretion of gastrin by 48%, cholecystokinin by 82% and pancreatic polypeptide by 107%. These results point to considerable qualitative and quantitative differences in the stimulatory mechanisms of bombesin on the hormones studied.
注入神经肽蛙皮素会通过一种未知机制刺激几种胃肠激素的分泌。我们研究了阿托品（静脉推注15 ng/kg，随后以2.5 ng/kg持续30分钟）和生长抑素（静脉推注125微克，随后以62.5微克/30分钟持续给药）对6名健康志愿者中60 pmol/kg×20分钟蛙皮素刺激3种激素（胃泌素、胆囊收缩素和胰多肽）分泌的影响。在-5、0、2.5、5、7.5、10、15、20、25和30分钟采集血浆样本，通过灵敏且特异的放射免疫分析法测定激素。蛙皮素使血浆胃泌素显著升高（从12±2升至34±3 pM；P<0.0005）、胆囊收缩素显著升高（从1.2±0.2升至8.9±0.7 pM；P<0.0001）以及胰多肽显著升高（从22±4升至72±19 pM；P<0.05）。阿托品和生长抑素对蛙皮素激素反应的影响存在很大差异。阿托品使胃泌素反应轻微升高19%，使胆囊收缩素反应升高15%，但强烈抑制蛙皮素刺激的胰多肽分泌达97%。另一方面，生长抑素抑制蛙皮素诱导的胃泌素分泌48%、胆囊收缩素分泌82%以及胰多肽分泌107%。这些结果表明蛙皮素对所研究激素的刺激机制在质和量上存在相当大的差异。
