Liu Yuan, Wang Wenbo, Zou Ziying, Fan Quanshui, Hu Zonghai, Feng Ziliang, Zhu Bing, Xiong Jie
Clinical laboratory, General Hospital of Chengdu Military Region of PLA, Chengdu, China.
Center for Disease Control and Prevention of Chengdu Military Region, Chengdu, China.
Virus Res. 2017 Aug 15;240:190-196. doi: 10.1016/j.virusres.2017.08.013. Epub 2017 Aug 30.
Hepatitis C Virus (HCV) infection usually progress to chronic liver disease and shows a significant increase in total monocyte/macrophages numbers in the liver. Monocyte chemoattractant protein-1 (MCP-1) plays a role in the recruitment of monocytes to the liver. In this study we found that MCP-1 were up-regulated in macrophages cultured with cell-culture derived infectious HCV particles (HCVcc) and promoted the migration of monocytes. IL1β, IL6 and TNFα were factors that induced MCP-1 expression, which were up-regulated in macrophages induced by HCV. Long-term of HCV incubation induced apoptosis of macrophages. Finally, we observed the effect of HCV infected macrophages on nearby liver cells. Huh7 cells continuously co-cultured with monocyte/macrophages displayed increased expression of pro-inflammatory cytokines and the morphology of Huh7 cells were greatly changed. Taken together, our study provides more information for the role of monocyte/macrophages in HCV related chronic liver disease.
丙型肝炎病毒（HCV）感染通常会进展为慢性肝病，且肝脏中的总单核细胞/巨噬细胞数量显著增加。单核细胞趋化蛋白-1（MCP-1）在单核细胞向肝脏的募集过程中发挥作用。在本研究中，我们发现MCP-1在用细胞培养衍生的传染性HCV颗粒（HCVcc）培养的巨噬细胞中上调，并促进单核细胞的迁移。IL1β、IL6和TNFα是诱导MCP-1表达的因子，它们在HCV诱导的巨噬细胞中上调。长期的HCV孵育诱导巨噬细胞凋亡。最后，我们观察了HCV感染的巨噬细胞对附近肝细胞的影响。与单核细胞/巨噬细胞持续共培养的Huh7细胞显示促炎细胞因子表达增加，且Huh7细胞的形态发生了很大变化。综上所述，我们的研究为单核细胞/巨噬细胞在HCV相关慢性肝病中的作用提供了更多信息。
