腱调蛋白与雷珠单抗对血管生成的抑制作用。
Inhibitory effect of tenomodulin versus ranibizumab on angiogenesis.
作者信息
Wang Wei, Liu Guang-Xu, Li Yue-Hua, Li Xue-Dong, He Yan
机构信息
Department of Ophthalmology, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, China.
Department of Epidemiology and Health Statistics, School of Public Health, Capital Medical University, Beijing 100069, China.
出版信息
Int J Ophthalmol. 2017 Aug 18;10(8):1212-1216. doi: 10.18240/ijo.2017.08.04. eCollection 2017.
AIM
To evaluate anti-angiogenic effect of tenomodulin (TNMD) and ranibizumab on cell proliferation and capillary-like morphogenesis of vascular endothelial cells under the stimulation of vascular endothelial growth factor (VEGF)
METHODS
The effects of TNMD and ranibizumab on VEGF-induced proliferation of human umbilical vein endothelial cells (HUVECs) were evaluated by MTT assay, and the effects of TNMD and ranibizumab on capillary-like structures formed by HUVECs under the stimulation of VEGF were examined in culture. Capillary-like morphogenesis of HUVECs was quantitatively evaluated, and total lengths of tube-like structures per field were measured in a masked way.
RESULTS
HUVECs with both ranibizumab and TNMD protein showed MTT reduction in VEGF-stimulated cell proliferation as expected, while MTT absorbance in the HUVECs with TNMD was significantly declined than that with ranibizumab (<0.01). The capillary-like structures formed by HUVECs were markedly impaired by the presence of both TNMD and ranibizumab in the culture medium. The total length of the capillary-like structures per field was significantly shorter in the medium with TNMD than that of ranibizumab (<0.01). The inhibitory effect of TNMD on tube formation angiogenesis was significantly stronger than that of ranibizumab.
CONCLUSION
TNMD may have stronger inhibitory effect than ranibizumab on angiogenesis.
目的
评估腱调蛋白（TNMD）和雷珠单抗对血管内皮生长因子（VEGF）刺激下血管内皮细胞增殖及毛细血管样形态形成的抗血管生成作用
方法
采用MTT法评估TNMD和雷珠单抗对VEGF诱导的人脐静脉内皮细胞（HUVECs）增殖的影响，并在培养中检测TNMD和雷珠单抗对VEGF刺激下HUVECs形成的毛细血管样结构的影响。对HUVECs的毛细血管样形态形成进行定量评估，并以盲法测量每个视野中管状结构的总长度。
结果
正如预期的那样，雷珠单抗和TNMD蛋白处理的HUVECs在VEGF刺激的细胞增殖中MTT值降低，而TNMD处理的HUVECs的MTT吸光度比雷珠单抗处理的显著下降（<0.01）。培养基中同时存在TNMD和雷珠单抗会显著损害HUVECs形成的毛细血管样结构。含有TNMD的培养基中每个视野的毛细血管样结构总长度显著短于含有雷珠单抗的培养基（<0.01）。TNMD对管形成血管生成的抑制作用明显强于雷珠单抗。
结论
TNMD对血管生成的抑制作用可能比雷珠单抗更强。
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