Mai Keith Ka Ki, Kang Byung-Ho
State Key Laboratory of Agrobiotechnology, Centre for Cell and Developmental Biology, School of Life Sciences, Chinese University of Hong Kong, Hong Kong, China.
Methods Mol Biol. 2017;1662:97-104. doi: 10.1007/978-1-4939-7262-3_8.
Electron tomography is a powerful tool for visualizing subcellular organelles. With the advances in cryo-fixation techniques, it is now possible to reconstruct complex structures in cells preserved close to their native states in three-dimension (3D) using electron tomography. In order to better visualize these objects, 3D models are made from outlines of organelles in individual tomographic slices, which can be used to display morphological features and quantify structural parameters. While outlines of simple organelles can be drawn by hand fairly quickly, it is possible to accelerate 3D modeling of more complex organelles by means of semiautomatic segmentation. In this chapter, we use the example of reconstructing Golgi cisternae of a plant cell into 3D models using the semiautomatic protocol.
电子断层扫描是一种用于可视化亚细胞器的强大工具。随着冷冻固定技术的进步，现在可以使用电子断层扫描在三维（3D）中重建接近其天然状态保存的细胞中的复杂结构。为了更好地可视化这些物体，3D模型由单个断层切片中的细胞器轮廓制成，可用于显示形态特征和量化结构参数。虽然简单细胞器的轮廓可以相当快速地手动绘制，但通过半自动分割可以加速更复杂细胞器的3D建模。在本章中，我们以使用半自动协议将植物细胞的高尔基体池重建为3D模型为例。
