Wu Boyue, Ouyang Zhengyu, Lyon Christopher J, Zhang Wei, Clift Tori, Bone Christopher R, Li Boan, Zhao Zhen, Kimata Jason T, Yu Xu G, Hu Ye
Biodesign Center for Personalized Diagnostics, the Biodesign Institute, Arizona State University , 727 E. Tyler Street, Tempe, Arizona 85281, United States.
College of Laboratory Medicine, Tianjin Medical University , 1 Guangdong Road, Tianjin 300203, China.
ACS Infect Dis. 2017 Dec 8;3(12):880-885. doi: 10.1021/acsinfecdis.7b00042. Epub 2017 Sep 15.
Individuals who exhibit long-term HIV suppression and CD4 T-cell preservation without antiretroviral therapy are of great interest for HIV research. There is currently no robust method for rapid identification of these "HIV controller" subjects; however, HLA-B57 (human leukocyte antigen (major histocompatibility complex), class I, B57) genotype exhibits modest sensitivity for this phenotype. Complement C3b and C4b can influence HIV infection and replication, but studies have not examined their possible link to HIV controller status. We analyzed HLA-B*57 genotype and complement levels in HIV-positive patients receiving suppressive antiretroviral therapy, untreated HIV controllers, and HIV-negative subjects to identify factors associated with HIV controller status. Our results revealed that the plasma levels of three C4b-derived peptides and complement factor I outperformed all other assayed biomarkers for HIV controller identification, although we could not analyze the predictive value of biomarker combinations with the current sample size. We believe this rapid screening approach may prove useful for improved identification of HIV controllers.
在未接受抗逆转录病毒治疗的情况下表现出长期HIV抑制和CD4 T细胞保存的个体对HIV研究具有重大意义。目前尚无快速识别这些“HIV控制者”受试者的可靠方法；然而，HLA - B57（人类白细胞抗原（主要组织相容性复合体），I类，B57）基因型对该表型表现出一定的敏感性。补体C3b和C4b可影响HIV感染和复制，但研究尚未考察它们与HIV控制者状态之间的可能联系。我们分析了接受抑制性抗逆转录病毒治疗的HIV阳性患者、未经治疗的HIV控制者以及HIV阴性受试者的HLA - B*57基因型和补体水平，以确定与HIV控制者状态相关的因素。我们的结果显示，三种C4b衍生肽和补体因子I的血浆水平在识别HIV控制者方面优于所有其他检测的生物标志物，尽管以目前的样本量我们无法分析生物标志物组合的预测价值。我们认为这种快速筛查方法可能有助于更好地识别HIV控制者。
