Dong Hua-Jiang, Shang Chong-Zhi, Li Gang, Niu Qing, Luo Yue-Chen, Yang Yue, Meng Hui-Peng, Yin Hui-Juan, Zhang Hai-Xia, Zhao Ming-Liang, Lin Ling
*State Key Laboratory of Precision Measurement Technology and Instruments, Tianjin University †Tianjin Key Laboratory of Neurotrauma Repair, Neurological Hospital, Affiliated Hospital of Logistics University of Chinese People's Armed Police Forces ‡State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College §The Biomedical Engineering Institute of the Chinese Academy of Medical Sciences, Tianjin, China.
J Craniofac Surg. 2017 Sep;28(6):1615-1619. doi: 10.1097/SCS.0000000000003563.
The authors aim to track the distribution of human umbilical cord mesenchymal stem cells (MSCs) in large blood vessel of traumatic brain injury -rats through immunohistochemical method and small animal imaging system. After green fluorescent protein (GFP) gene was transfected into 293T cell, virus was packaged and MSCs were transfected. Mesenchymal stem cells containing GFP were transplanted into brain ventricle of rats when the infection rate reaches 95%. The immunohistochemical and small animal imaging system was used to detect the distribution of MSCs in large blood vessels of rats. Mesenchymal stem cells could be observed in large vessels with positive GFP expression 10 days after transplantation, while control groups (normal group and traumatic brain injury group) have negative GFP expression. The vascular endothelial growth factor in transplantation group was higher than that in control groups. The in vivo imaging showed obvious distribution of MSCs in the blood vessels of rats, while no MSCs could be seen in control groups. The intravascular migration and homing of MSCs could be seen in rats received MSCs transplantation, and new angiogenesis could be seen in MSCs-transplanted blood vessels.
作者旨在通过免疫组织化学方法和小动物成像系统追踪人脐带间充质干细胞（MSCs）在创伤性脑损伤大鼠大血管中的分布。将绿色荧光蛋白（GFP）基因转染入293T细胞后，包装病毒并转染MSCs。当感染率达到95%时，将含GFP的间充质干细胞移植到大鼠脑室。采用免疫组织化学和小动物成像系统检测大鼠大血管中MSCs的分布。移植后10天，在大血管中可观察到GFP表达阳性的间充质干细胞，而对照组（正常组和创伤性脑损伤组）GFP表达为阴性。移植组血管内皮生长因子高于对照组。体内成像显示大鼠血管中有明显的MSCs分布，而对照组未见MSCs。在接受MSCs移植的大鼠中可见MSCs的血管内迁移和归巢，在移植MSCs的血管中可见新生血管形成。
