Aldhaher Areej, Langat Moses, Ndunda Beth, Chirchir Denis, Midiwo Jacob O, Njue Alice, Schwikkard Sianne, Carew Mark, Mulholland Dulcie
Natural Products Research Group, Department of Chemistry, Faculty of Engineering and Physical Sciences, University of Surrey, Guildford, GU2 7XH, United Kingdom; Department of Biology, College of Science, Basrah University, Basrah, Iraq.
Natural Products Research Group, Department of Chemistry, Faculty of Engineering and Physical Sciences, University of Surrey, Guildford, GU2 7XH, United Kingdom; School of Chemistry and Physics, University of Natal, Durban, 4041, South Africa.
Phytochemistry. 2017 Dec;144:1-8. doi: 10.1016/j.phytochem.2017.08.014. Epub 2017 Sep 1.
Four previously undescribed diterpenoids including two crotofolanes, crotodichogamoin A and B, and two halimanes, crothalimene A and B, a new sesquiterpenoid, and fifteen previously reported compounds, including the crotofolane, crotohaumanoxide, the casbane, depressin, a further seven furanohalimane diterpenoids, three patchoulane and two further cadinane sesquiterpenoids and aleuritolic acid were isolated from the root of Croton dichogamus. Crotodichogamoin B is an important biosynthetic intermediate of the crotofolane class and this is the first report of patchoulene sesquiterpenoids from the genus. Compounds were tested at one concentration, 1 × 10 M, in the NCI59 cell one-dose screen but did not show significant activity snd were also evaluated for their cytotoxicity against Caco-2 cell lines using the neutral red assay. 10-epi-Maninsigin D reduced Caco-2 cell viability at 10, 30 and 100 μM, with values of decreased viability of 28%, 48% and 43% respectively. None of the other tested compounds showed significant activity.
从毛果巴豆的根中分离出4个前所未有的二萜类化合物，包括2个巴豆叶烷类化合物巴豆二乔素A和B，以及2个哈利烷类化合物巴豆哈利烯A和B，1个新的倍半萜类化合物，还有15个先前报道过的化合物，包括巴豆叶烷、巴豆人氧化素、卡斯巴烷、去甲降香萜烯，另外7个呋喃哈利烷二萜类化合物、3个广藿香烷和另外2个杜松烷倍半萜类化合物以及紫铆醇酸。巴豆二乔素B是巴豆叶烷类化合物的重要生物合成中间体，这是该属中广藿香烯倍半萜类化合物的首次报道。化合物在NCI59细胞单剂量筛选中以1×10⁻⁶M的浓度进行测试，但未显示出显著活性，并且还使用中性红试验评估了它们对Caco-2细胞系的细胞毒性。10-表-马尼西格因D在10、30和100μM时降低了Caco-2细胞活力，活力降低值分别为28%、48%和43%。其他测试化合物均未显示出显著活性。
