University of California San Diego, Reproductive Medicine, La Jolla, CA, United States.
University of California San Diego, Reproductive Medicine, La Jolla, CA, United States; University of Calgary, Comparative Biology & Experimental Medicine, Calgary, AB, Canada.
Placenta. 2017 Sep;57:170-174. doi: 10.1016/j.placenta.2017.06.343. Epub 2017 Jul 6.
Glucose metabolism in trophoblast cells is essential to provide the required energy for the development and function of the placenta. Glyceraldehyde 3-phosphate dehydrogenase (Gapdh), a key enzyme in the glycolysis pathway has been considered ubiquitously expressed in cells. There is, however, a growing body of evidence suggesting that Gapdh has many functions in pathways unrelated to glucose metabolism. In the present study, we show that GAPDH expression and sub-cellular localization changes through gestation in the mouse placenta. Our findings raise the possibility that GAPDH has multiple functions in trophoblast cells and the developing placenta, while also cautioning against its use as an endogenous reference or standard for gene expression in the placenta.
滋养层细胞中的葡萄糖代谢对于为胎盘的发育和功能提供所需的能量至关重要。甘油醛-3-磷酸脱氢酶（Gapdh）是糖酵解途径中的关键酶，被认为在细胞中广泛表达。然而，越来越多的证据表明，Gapdh 在与葡萄糖代谢无关的途径中具有多种功能。在本研究中，我们显示 GAPDH 在小鼠胎盘的妊娠过程中表达和亚细胞定位发生变化。我们的研究结果提出了一种可能性，即 GAPDH 在滋养层细胞和发育中的胎盘中有多种功能，同时也警告不要将其用作胎盘基因表达的内源性参考或标准。
