Aszyk Justyna, Kubica Paweł, Kot-Wasik Agata, Namieśnik Jacek, Wasik Andrzej
Department of Analytical Chemistry, Faculty of Chemistry, Gdańsk University of Technology,11/12 Narutowicza Street, 80-233 Gdańsk, Poland.
Department of Analytical Chemistry, Faculty of Chemistry, Gdańsk University of Technology,11/12 Narutowicza Street, 80-233 Gdańsk, Poland.
J Chromatogr A. 2017 Oct 13;1519:45-54. doi: 10.1016/j.chroma.2017.08.056. Epub 2017 Aug 25.
Liquid chromatography-tandem mass spectrometry with electrospray ionization (HPLC-ESI-MS/MS) methods were developed for the simultaneous determination of 42 flavouring compounds and nicotine in liquids for e-cigarettes. The chromatographic separation was performed using an Ace Ultracore™ SuperC18™ (100×2.1mm, 2.5μm) column in both acidic and alkaline pH conditions to separate all the compounds. A simple "dilute & shoot" approach was used for the sample preparation. The method validation was performed by evaluating key analytical parameters such as linearity, accuracy, selectivity, precision, limit of detection (LOD) and limit of quantification (LOQ). The calibration curves showed good linearity within the specific ranges for the investigated compounds with correlation coefficients greater than 0.990 in each case. The recovery for all the investigated compounds varied from 89% to 110%. The intra- and inter-day precision were within the acceptable limits (±15%) at all tested concentrations. The applicability of the methods was examined by analysing 25 liquid samples from e-cigarettes commercially available on the Polish market.
建立了液相色谱-电喷雾串联质谱法（HPLC-ESI-MS/MS），用于同时测定电子烟烟液中的42种调味化合物和尼古丁。在酸性和碱性pH条件下，使用Ace Ultracore™ SuperC18™（100×2.1mm，2.5μm）色谱柱进行色谱分离，以分离所有化合物。采用简单的“稀释进样”方法进行样品制备。通过评估线性、准确度、选择性、精密度、检测限（LOD）和定量限（LOQ）等关键分析参数来进行方法验证。校准曲线在所研究化合物的特定范围内显示出良好的线性，每种情况下相关系数均大于0.990。所有研究化合物的回收率在89%至110%之间。在所有测试浓度下，日内和日间精密度均在可接受范围内（±15%）。通过分析从波兰市场上购买的25种电子烟烟液样品，检验了该方法的适用性。
