National Centre for Chemicals, Istituto Superiore di Sanità, Rome, Italy.
Department of Biology, University of Bari "Aldo Moro", Bari, Italy.
J Sep Sci. 2017 Mar;40(5):1049-1056. doi: 10.1002/jssc.201601076. Epub 2017 Jan 31.
A liquid chromatography with tandem mass spectrometry method for the simultaneous quantification of nicotine and seven minor tobacco alkaloids in both refill liquids for electronic cigarettes and their generated aerosol was developed and validated. The limit of detection and limit of quantification values were 0.3-20.0 and 1.0-31.8 ng/mL, respectively. Within-laboratory reproducibility was 8.2-14.2% at limit of quantification values and 4.8-12.7% at other concentration levels. Interday recovery was 75.8-116.4%. The method was applied to evaluate the compliance of commercial liquids (n = 95) with their labels and to assess levels of minor alkaloids. Levels of nicotine and its corresponding compounds were also evaluated in generated aerosol. About 47% of samples showed differences above ±10 % of the stated nicotine concentration. About 78% of the "zero nicotine" liquids showed traces in the range of 1.3 ± 0.1-254.0 ± 14.6 μg/mL. Nicotine-N'-oxides, myosmine, and anatabine were the most common minor alkaloids in liquids containing nicotine. Nicotine and N'-oxides were detected in all air samples when aerosol was generated from liquids containing nicotine. Nicotine average emissions from electronic cigarette (2.7 ± 0.9 μg/m ) were significantly lower (p < 0.01, t-test) with respect to conventional cigarette (30.2 ± 1.5 μg/m ).
建立并验证了一种同时测定电子烟烟液及其产生气溶胶中尼古丁和七种微量烟草生物碱的液相色谱-串联质谱法。检测限和定量限分别为 0.3-20.0 和 1.0-31.8ng/mL。在定量限浓度水平下，实验室内部重现性为 8.2-14.2%，在其他浓度水平下为 4.8-12.7%。日内回收率为 75.8-116.4%。该方法用于评估商业液体（n=95）与标签的一致性，并评估微量生物碱的水平。还评估了生成气溶胶中尼古丁及其相应化合物的水平。约 47%的样品显示出与标签上尼古丁浓度相差±10%以上的差异。约 78%的“无尼古丁”液体在 1.3±0.1-254.0±14.6μg/mL 的范围内显示出痕迹。尼古丁-N-氧化物、肌菔碱和新烟草碱是含有尼古丁的液体中最常见的微量生物碱。当从含有尼古丁的液体中产生气溶胶时，所有空气样本中都检测到尼古丁和 N-氧化物。电子烟（2.7±0.9μg/m）的尼古丁平均排放量显著低于（p<0.01，t 检验）传统香烟（30.2±1.5μg/m）。
