United States Department of Agriculture, Animal and Plant Health Inspection Service, Wildlife Services, National Wildlife Research Center, 4101 LaPorte Avenue, Fort Collins, CO 80521, USA.
Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Science, Colorado State University, 300 W Drake Road, Fort Collins, CO 80525, USA.
Fish Shellfish Immunol. 2017 Dec;71:255-263. doi: 10.1016/j.fsi.2017.08.038. Epub 2017 Aug 31.
An experimental contraceptive vaccine was evaluated in Atlantic salmon (Salmo salar). A peptide derived from the beta subunit of luteinizing hormone (LH) was conjugated to two different carrier proteins, bovine serum albumin (BSA) and keyhole limpet hemocyanin (KLH), and formulated with one of four immunostimulants in a water-in-oil emulsion. Specific antibody responses to the peptide and each carrier protein were evaluated. While the antibody response to KLH was stronger than the response to BSA, both carrier proteins stimulated comparable antibody responses to the LH peptide. The immunostimulant proved to be more important for enhancing the LH peptide antibody response than the carrier protein selection; vaccines containing a combination of Aeromonas salmonicida and Vibrio anguillarum stimulated significantly greater LH peptide antibody production than any of the other three immunostimulants evaluated at 12 weeks post-vaccination. This study provides proof-of-concept for specific antibody production against a hapten-carrier protein antigen in Atlantic salmon and reinforces the importance of vaccine immunostimulant selection.
一种实验性避孕疫苗在大西洋鲑鱼（Salmo salar）中进行了评估。一种从促黄体激素（LH）β亚基衍生而来的肽与两种不同的载体蛋白牛血清白蛋白（BSA）和血蓝蛋白（KLH）偶联，并与四种免疫佐剂中的一种在水包油乳液中配制。评估了针对该肽和每种载体蛋白的特异性抗体反应。尽管 KLH 的抗体反应强于 BSA，但两种载体蛋白均刺激了对 LH 肽的类似抗体反应。免疫佐剂对于增强 LH 肽抗体反应比载体蛋白选择更为重要；与在疫苗中评估的其他三种免疫佐剂相比，含有嗜水气单胞菌和鳗弧菌的疫苗在接种后 12 周时能显著增加 LH 肽抗体的产生。这项研究为大西洋鲑鱼针对半抗原-载体蛋白抗原产生特异性抗体提供了概念验证，并强调了疫苗免疫佐剂选择的重要性。
