Chalmers Lynn, Thompson Kim D, Taylor John F, Black Sean, Migaud Herve, North Ben, Adams Alexandra
Institute of Aquaculture, University of Stirling, Stirling, FK9 4LA, UK.
Institute of Aquaculture, University of Stirling, Stirling, FK9 4LA, UK; Moredun Research Institute, Pentlands Science Park, Bush Loan, Midlothian, EH26 0PZ, UK.
Fish Shellfish Immunol. 2016 Oct;57:301-308. doi: 10.1016/j.fsi.2016.08.049. Epub 2016 Aug 25.
Sterile triploid fish represent a solution to the problems associated with sexual maturation and escapees in aquaculture. However, as disease outbreaks continue to cause significant economic losses to the industry, it is essential that the response of triploids to disease and disease treatments be characterised. The aim of this study was to compare the response of triploid Atlantic salmon to a commercial furunculosis vaccine with that of diploid fish, and to assess the vaccine efficacy in the two ploidies through an experimental infection with Aeromonas salmonicida. Diploid and triploid Atlantic salmon were injected intraperitoneally with either phosphate buffered saline, liquid paraffin adjuvant or a commercial furunculosis vaccine. Following vaccination, growth, adhesion scores and a variety of assays to assess immune function, such as respiratory burst and antibody response, were measured. Vaccination did not have a significant effect on the weight of either ploidy prior to challenge at 750° days. Adhesion scores were significantly higher in vaccinated fish compared to unvaccinated fish, although no effect of ploidy was observed. Ploidy significantly affected respiratory burst activity following vaccination, however, with triploids exhibiting higher activity than diploids. Combined with lower white blood cell numbers observed in the triploids, it may be that this low cell number is compensated for by increased cellular activity. Ploidy however, did not have a significant effect on complement activity or antibody response, with significantly higher antibody levels detected in all vaccinated fish compared to unvaccinated controls. In addition, both ploidy groups were well protected following challenge with no difference in the relative percentage survival. Based on these results, it appears that ploidy does not affect the severity of adhesions that result post-vaccinate or in the fish's immune response following vaccination, and the furunculosis vaccine performs equally well in both diploid and triploid Atlantic salmon.
无菌三倍体鱼为解决水产养殖中性成熟和逃逸相关问题提供了一种方案。然而，由于疾病爆发持续给该行业造成重大经济损失，明确三倍体对疾病及疾病治疗的反应至关重要。本研究的目的是比较三倍体大西洋鲑与二倍体鱼对一种商业性疖疮病疫苗的反应，并通过用杀鲑气单胞菌进行实验性感染来评估两种倍性鱼的疫苗效力。将二倍体和三倍体大西洋鲑腹腔注射磷酸盐缓冲盐水、液体石蜡佐剂或一种商业性疖疮病疫苗。接种疫苗后，测量生长情况、黏附评分以及各种评估免疫功能的测定指标，如呼吸爆发和抗体反应。在750度日进行攻毒前，接种疫苗对两种倍性鱼的体重均无显著影响。与未接种疫苗的鱼相比，接种疫苗的鱼黏附评分显著更高，不过未观察到倍性的影响。然而，倍性对接种疫苗后的呼吸爆发活性有显著影响，三倍体的活性高于二倍体。结合三倍体中观察到的白细胞数量较低的情况，可能是这种低细胞数量通过细胞活性增加得到了补偿。然而，倍性对补体活性或抗体反应没有显著影响，与未接种疫苗的对照组相比，所有接种疫苗的鱼检测到的抗体水平显著更高。此外，两个倍性组在攻毒后都得到了良好的保护，相对存活率没有差异。基于这些结果，似乎倍性不影响接种疫苗后产生的黏附严重程度或接种疫苗后鱼的免疫反应，并且疖疮病疫苗在二倍体和三倍体大西洋鲑中表现同样良好。
