Mass Spectrometry Engineering Technology Research Center, Center for Advanced Measurement Science, National Institute of Metrology, Beijing, 100029, People's Republic of China.
Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, People's Republic of China.
Mikrochim Acta. 2019 Feb 18;186(3):193. doi: 10.1007/s00604-019-3255-6.
Pyrenylbutyric acid and streptavidin were coupled to films of reduced graphene oxide (rGO) and then conjugated to a biotinylated broad-spectrum monoclonal antibody against aflatoxins (AFs). It is shown that such films can efficiently and selectively capture AFs inculding AFB, AFB, AFG, AFG, AFM and AFM. The rGO films were characterized by using scanning electron microscopy, energy-dispersive spectroscopy, and raman spectroscopy. The selectivity and purification performance of the antibody-loaded rGO films were investigated. They were applied to the purification of extremely small samples (100 μL) of AFs-spiked rabbit serum after enzymatic hydrolysis. The AFs were analyzed by ultra-performance liquid chromatography coupled to tandem mass spectrometry. The limits of detection for the six AFs investigated ranged from 50 to 170 pg·mL. The average recoveries of AFs in spiked rabbit serum samples ranged from 55% to 75%, with relative standard deviations of less than 9.4%. Graphical abstract Design of a multifunctional sandwich film that consists of a reduced graphene oxide film base, a pyrenylbutyric acid middle layer and a broad-specificity anti-AF monoclonal antibody surface layer. It was successfully applied to the determination of aflatoxins in only 100 μL of rabbit serum samples with satisfactory selectivity and acceptable accuracy.
芘丁酸和链霉亲和素偶联到还原氧化石墨烯（rGO）薄膜上，然后与生物素化的广谱抗黄曲霉毒素单克隆抗体结合。结果表明，这种薄膜可以有效地和选择性地捕获黄曲霉毒素，包括 AFB1、AFB2、AFG1、AFG2、AFM1 和 AFM2。使用扫描电子显微镜、能量色散光谱和拉曼光谱对 rGO 薄膜进行了表征。研究了载抗体 rGO 薄膜的选择性和纯化性能。将其应用于酶解后极少量（100 μL）黄曲霉毒素污染兔血清的纯化。通过超高效液相色谱-串联质谱法分析黄曲霉毒素。六种黄曲霉毒素的检测限范围为 50 至 170 pg·mL。在加标兔血清样品中，黄曲霉毒素的平均回收率为 55%至 75%，相对标准偏差小于 9.4%。
