Hu Yang-Shan, Ren Tian-Heng, Li Zhi, Tang Ying-Zi, Ren Zheng-Long, Yan Ben-Ju
College of Life Science, Sichuan Agricultural University, Ya'an 625014, Sichuan, China.
Agronomy College, Sichuan Agricultural University, Chengdu 611130, Sichuan, China.
Gene. 2017 Nov 15;634:15-21. doi: 10.1016/j.gene.2017.08.039. Epub 2017 Sep 1.
Spike formation rate (SR), which is based on maximum tiller number per unit area and spike number per unit area, is an important yield-related trait in wheat. Increasing the spike formation rate reduces growth competition and wastage of photosynthate from ineffective tillers. Unfortunately, research studies involving quantitative trait locus (QTL) mapping for wheat spike formation rate are limited. In the present study, a set of 371 recombinant inbreed line (RIL) population, which were derived from 1BL/LRS wheat-rye translocation lines CN18 and T1208, was analysed by simple sequence repeat (SSR) markers. Genetic analysis showed that a stable and major QTL (QSR.sicau-4D) for spike formation rate was localized to chromosome 4D and explained 18.24% and 24.48% of the observed phenotypic variance in 2015 and 2016, respectively. This QTL was closely linked to SSR marker Xcfd23, and the genetic distance between the flank markers was 3.28cM. Furthermore, QSR.sicau-4D might be a novel pleiotropic QTL, which also controlled maximum tiller number per unit area (QMTN.sicau-4D) and tiller number during pre-winter per unit area (QTNW.sicau-4D). The marker Xcfd23 associated with SR may be utilized in marker-assisted selection in wheat breeding.
穗形成率（SR）基于单位面积的最大分蘖数和单位面积的穗数，是小麦中一个重要的产量相关性状。提高穗形成率可减少无效分蘖的生长竞争和光合产物的浪费。遗憾的是，涉及小麦穗形成率数量性状位点（QTL）定位的研究有限。在本研究中，利用简单序列重复（SSR）标记对一组由1BL/1RS小麦-黑麦易位系CN18和T1208衍生而来的371个重组自交系（RIL）群体进行了分析。遗传分析表明，一个稳定的穗形成率主效QTL（QSR.sicau-4D）定位于4D染色体，分别解释了2015年和2016年观测表型变异的18.24%和24.48%。该QTL与SSR标记Xcfd23紧密连锁，侧翼标记间的遗传距离为3.28cM。此外，QSR.sicau-4D可能是一个新的多效性QTL，它还控制单位面积的最大分蘖数（QMTN.sicau-4D）和越冬前单位面积的分蘖数（QTNW.sicau-4D）。与穗形成率相关的标记Xcfd23可用于小麦育种的标记辅助选择。
