The State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Cotton Science Research Institute of Hunan Province, Changde 415101, Hunan, China.
Int J Mol Sci. 2017 Aug 30;18(9):1874. doi: 10.3390/ijms18091874.
RNA interference (RNAi) has been developed as an efficient technology. RNAi insect-resistant transgenic plants expressing double-stranded RNA (dsRNA) that is ingested into insects to silence target genes can affect the viability of these pests or even lead to their death. , a molt-regulating transcription factor gene, was previously selected as a target expressed in bacteria and tobacco plants to control by RNAi technology. In this work, we selected the dsRNA- fragment to silence in cotton bollworm for plant mediated-RNAi research. A total of 19 transgenic cotton lines expressing were successfully cultivated, and seven generated lines were used to perform feeding bioassays. Transgenic cotton plants expressing ds were shown to induce high larval mortality and deformities of pupation and adult eclosion when used to feed the newly hatched larvae, and 3rd and 5th instar larvae of . Moreover, transgenic cotton also demonstrated an improved cotton yield when compared with controls.
RNA 干扰 (RNAi) 已发展成为一种有效的技术。表达双链 RNA (dsRNA) 的 RNAi 抗虫转基因植物被昆虫摄入以沉默靶基因，这可能会影响这些害虫的生存能力，甚至导致它们死亡。蜕皮调节转录因子基因以前被选为在细菌和烟草植物中表达的目标，以通过 RNAi 技术控制棉铃虫。在这项工作中，我们选择了 dsRNA- 片段来沉默棉铃虫中的 ，用于植物介导的-RNAi 研究。成功培育了表达 的 19 个转基因棉花品系，其中 7 个生成品系用于进行喂食生物测定。结果表明，当用于喂养刚孵化的幼虫以及第 3 和第 5 龄幼虫时，表达 ds 的转基因棉花植物会诱导高幼虫死亡率和蛹化畸形以及成虫羽化，此外，与对照相比，转基因棉花还表现出提高的棉花产量。
